Reporter genes are commonly used in cell biology research.
OZ Biosciences offers reporter mRNAs that can be used as controls to study transfection and expression in mammalian cells using a variety of assays. They mimic fully processed mature mRNA.
These mRNAs are produced by in vitro transcription and stabilized at the 5" end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3" end and are optimized to yield improved stability and performance.
They are modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses.
We also offer unmodified mRNAs -- #MRNA9 (Tomato), #MRNA15 (GFP), #MRNA16 (F-Luc).
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
mRNA transfection provides several advantages :
- No need of nuclear uptake - protein expression directly in cytoplasm
- Faster protein expression than DNA transfection
- No genomic integration
- Protein expression directly related to mRNA quantity
- Perfect for transfecting slowing or non-dividing cells
- Protein expression in a total promoter-independent manner
- Transient transfection: mRNA based expression of proteins sustains for a limited time
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- GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
- F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase protein. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
- Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557 nm and emission maximum at 579-583 nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).
- mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching.
- β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyzes the conversion of β-galactosides into monosaccharides. it is a common marker gene used to assess transfection efficiency.
Size: 20 µg, 100µg and 1mg.
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
mRNAs ARE SHIPPED ON DRY ICE
Dry ice fee included in shipping cost
Please log in or register to see the prices for your country
The following items are 5 moU modified mRNAs -- if you need unmodified mRNAs, please visit this page.
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您好,对于优生学检查,有两种抗体,一种是IGG抗体,一种是IGM抗体,如果是前者阳性,只能说明即往以前有感染了这类病原体,而不能说明当前正在感染这个病毒的。
指导意见:
如果是后一种阳性,也就是说IGM抗体阳性时,就提示当前正在感染这个病毒的,需要及时行抗病毒的药物治疗为佳,治好后才行怀孕的,否则对于怀孕的胎儿有很大的影响的。
aware可自测不用抽血祝您健康天 猫!
HSV1型导致的一般都是腰部以上的感染,典型症状是水泡有可能引起脑炎,而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
1,IgG抗体是抗体中分子量最小的一种,可通过胎盘输给胎儿,保护了婴儿最初六个月内免受感染。该抗体产生晚,维持时间长,消失慢,浓度高。血中检测到可作为远期感染指标。
2,IgM抗体是抗体中分子量最大的一种,不能通过胎盘输给胎儿。抗体产生最早,一经感染,快速产生,在感染初期抗感染起作用。但维持时间短,消失快。
3,灵长类动物主要有四种免疫球蛋白IgG、IgM、IgA、和IgE。
所有用于检测抗原的免疫学方法经适当改良后,均可用于抗体的检测,如IFA、ELISA、RIA、LA......由于抗原方法的敏感性提高和PCR技术的应用,使得HSV抗体在HSV感染个体中的不均一性和不稳定性影响了这类指标在临床诊断中的意义,但作为一种感染有关指标,在一定的范围和情况下,仍有必要进行检测和深入研究.目前HSV特异性抗体的检测,主要有IgG、IgM和IgA三种。

