
Formulation | 20mMHepes,150mMNaCl,pH7.4 |
Storage | -80°C |
Purity | >95%bySDS-PAGE |
ActivityDetermination | FactorIXclottingassay |
ShelfLife(properlystored) | 12months |
TheactivationoffactorXbytheintrinsicfactorXasecomplexisshown.TheintrinsicfactorXasecomplexconsistsofanenzyme,factorIXa,andacofactor,factorVIIIa,assembledonaphospholipidsurfaceinthepresenceofcalciumions.Theenzymecomplexproteolyticallycleavesa10,000molecularweightactivationpeptidefromtheNH2-terminaloftheheavychainoffactorX,thusexpressingtheactive-siteoffactorXa.
SampleGelInformation:

Gel | Novex4-12%Bis-Tris |
---|---|
Load | HumanFactorIXa,1µgperlane |
Buffer | MOPS |
Standard | SeeBluePlus2;Myosin(191kDa),PhosphorylaseB(97kDa),BSA(64kDa),GlutamicDehydrogenase(51kDa),AlcoholDehydrogenase(39kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(19kDa),Lysozyme(14kDa) |
Overview:
FactorIXaisproducedfromitsinactiveprecursor,factorIX,viaproteolyticcleavagebyfactorXIaorthetissuefactor/factorVIIa/phospholipidcomplex.TheactivationresultsfromthecleavageoftwopeptidebondsinthefactorIXmolecule,releasinganactivationglycopeptidewithanapparentmolecularweightof10,000.TheheavychainoffactorIXa(Mr=28,000)containstheserineproteasecatalyticdomain,whilethelightchain(Mr=17,000)containsthemembranebindingdomain.
FactorIXafunctionsasaserineproteaseinvolvedintheactivationofthezymogen,factorX,toformtheenzyme,factorXa.ThefactorIXaenzymaticactivityisgreatlyenhancedbyinclusionofitscofactor,factorVIIIa,inthepresenceofcalciumionsonaphospholipidsurface.FactorIXaisreADIlyinhibitedbyantithrombinIII,andthisinhibitionisgreatlyacceleratedbythepresenceofheparin.FactorIXaisnotinhibitedbyDFP.
FactorIXaispreparedfromhighlypurifiedfactorIXbyactivationwithfactorXIa,asdescribedbyLindquistetal.(5).ThefactorIXaisfurtherpurifiedbygelfiltration,followedbyimmunoaffinitypurification.FactorIXaisalsoavailablewiththeactivesiteirreversIBLyblockedbythetripeptidechloromethylketone,EGRck,orbythefluorescentinhibitor,Dansyl-EGRck.Itissuppliedin50%(vol/vol)glycerol/H2Oforstorageat-20°C.PurityisassessedbySDS-PAGEanalysis.Activityisdeterminedinaone-stageclottingassay.
Properties:
Localization | Plasma | |||||
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Modeofaction | EnzymecomponentoftheFactorXasecomplex | |||||
Molecularweight | 45,000(7) | |||||
Extinctioncoefficient |
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Structure | 2subunits,Mr=28,000and17,000(5),NH2-terminalgla-domain,twoEGFdomains | |||||
Post-translationalmodifications | oneb-hydroxyaspartate(3),twelveglaresidues(4) |
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风疹病毒抗体IgM阳性才需要治疗。
HSV1型导致的一般都是腰部以上的感染,典型症状是水泡有可能引起脑炎,而且有非活化状态的潜伏。所以我觉得你根本就不用担心这个抗体IgG阳性的化验单。
IgM是免疫球蛋白M(Immunoglobulin M,IgM)的缩写。根据结构的不同将免疫球蛋白分为五种,IgM是人的免疫球蛋白之一,其他还有lgA、lgG、IgD和lgE。
文章原文链接http://www.jbc.org/content/282/23/16776.long

