货号： 3533-001-02 保存条件： -80°C 供应商： 深圳纽邦生物 数量： 2 规格： 1ml Cultrex®ReducedGrowthFactorBasementMembraneMatrix,Type2(BME2)
SelectanOptiontoSeePricingCultrex®RGFBMEType2(BME2)isaReducedGrowthFactorBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforrobustorganoidcultures.RGFBMEType2isanextracellularmatrixhydrogelthathasbeenusedextensivefortheestablishmentandexpansionoforganoids.RGFBMEType2mimicsthe invivo microenvironmenttoimprovetakerateandgrowthoforganoidprogenitorcells.
Description
Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.
Cultrex®RGFBMEType2isaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforrobustorganoidcultures.Cultrex®RGFBMEType2isprocessedtoreducematrix-associatedgrowthfactorsandprovideamoredefinedmodelsystem.
Qualitycontrolspecifications:
- Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
- Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
- PathClear®–testednegativebyPCRfor 17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- Proteinconcentrationof12–18mg/ml.
- NomycoplasmacontaminationdetectedbyPCR.
- ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
- Gelstabilitytestedforaperiodof14daysat37°C.
- Biologicalactivitydeterminedforeachlot:
- Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
- Organoidculture:BasementMembraneExtractpromotesgrowthandexpansionofmousesmallintestineorganoidprogenitorcells.
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