Accuris Taq DNA Polymerase and Master Mix
Accuris Taq DNA Polymerase provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher processivity and greater template affinity, resulting in consistently superior performance. Accuris Taq Polymerase exhibits a 5 to 3 nuclease activity, but no 3 to 5 (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.
· Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR.
· Improved template affinity and increased processivity for higher enzymatic activity and greater yields.
· Proprietary buffer optimized for a variety of assay conditions.
· Supplied with 5X buffer or as a convenient 2X Master Mix.
The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTPs not included). For convenience, Accuris Taq is also available in a ready to use 2X Master Mix - just add primers and template DNA. The Master Mix can be ordered with a red loading dye that allows samples to be loaded directly on an agarose gel.
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最近遇到了实验上的大问题，就是用一步法做RNA的荧光定量PCR时，发现低浓度的做不出来，而DNA是可以的，想请问各位在一步法PCR中有没有什么高见，就是逆转录的Buffer有没有什么需要注意的地方！就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer！
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内（外）起作用消化酶细胞外起作用B错误；
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误；
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确．
故选：D．
我是新手。我要开始做实验了，但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒，向好几家公司打了电话，但都没有我想要的东西。
没有这个东西，我的实验就只能是蓝图了。哪位战友有办法的，能不能帮帮我？
不胜感激。
请规范发贴，求助贴请在标题前加【求助】，谢谢您的合作。——by草根
