
Overview:
KAT7 or K (lysine) acetyltransferase 5 is a signaling protein that belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein which play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. KAT7 acts as a transcriptional inhibitor and inhibited AR-mediated transactivation of reporter constructs in CV-1 and PC-3 cells (1). KAT7 is part of a multisubunit complex that can acetylate histones H3 and H4 (2).
Gene Aliases:
KAT7; MYST2; HBO1; HBOA
Genbank Number:
NM_007067
References:
1. Sharma, M. et.al: Androgen receptor interacts with a novel MYST protein, HBO1. J. Biol. Chem. 275: 35200-35208, 2000. 2. Iizuka, M. et.al: Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein. J. Biol. Chem. 274: 23027-23034, 1999.
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最近遇到了实验上的大问题,就是用一步法做RNA的荧光定量PCR时,发现低浓度的做不出来,而DNA是可以的,想请问各位在一步法PCR中有没有什么高见,就是逆转录的Buffer有没有什么需要注意的地方!就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer!
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内(外)起作用消化酶细胞外起作用B错误;
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误;
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确.
故选:D.
我是新手。我要开始做实验了,但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒,向好几家公司打了电话,但都没有我想要的东西。
没有这个东西,我的实验就只能是蓝图了。哪位战友有办法的,能不能帮帮我?
不胜感激。
请规范发贴,求助贴请在标题前加【求助】,谢谢您的合作。——by草根

