PP2C, Protein Phosphatase 2C
PP2C Source: Bovine brain
Purity: > 90% by SDS-PAGE, apparent Mr ~ 45-kDa
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 1000 units/mg with casein as substrate. One unit is the amount of PP2C that releases 1 nmol of inorganic phosphate from 32P-labeled casein per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2C
GloboZymes PP2C preparations are useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and to evaluate the effects of test substances on the activity of the phosphatase.
Background: Protein Phosphatase 2C (PP2C) is one of the four major cytoplasmic protein serine/threonine phosphatases present in mammalian cells. It is a Mg2+-dependent protein phosphatase with a molecular mass of approximately 42 – 45 kDa. It regulates numerous cellular processes. For example, PP2C is involved in cell cycle control and the reversal of stress-activated protein kinase cascades.
Figure: SDS-PAGE pattern of a purified preparation of PP2C. The gel was stained with Coomassie Blue. The position of marker proteins is indicated.
Reference: Biochem J 287: 1019
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我是新手。我要开始做实验了，但一开始就碰到了个很大的问题。
我想买个能利用哺乳动物细胞RNA聚合酶将DNA转录成RNA的的质粒，向好几家公司打了电话，但都没有我想要的东西。
没有这个东西，我的实验就只能是蓝图了。哪位战友有办法的，能不能帮帮我？
不胜感激。
请规范发贴，求助贴请在标题前加【求助】，谢谢您的合作。——by草根
1970科家致癌RNA病毒发现种特殊RNA病毒聚合酶,该酶能RNA模板,根据碱基互补原则.程与般病毒转录向相反,故称逆转录,催化程酶称逆转录酶,发现哺乳物胚胎细胞裂淋巴细胞含.
逆转录病毒称携带逆转录酶病毒,先侵入宿主细胞病毒RNA模板,靠酶形DNA环化,合宿主细胞染色体原病毒形式宿主细胞代代传.
HIV（艾滋病病毒）典型逆转录病毒
