Overview
The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high-quality host genomic DNA, gram-positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum, and fecal samples. Elution volumes as low as 15 µL can be used to maintain higher nucleic acid concentrations.
The system combines Omega Bio-tek’s MicroElute columns with RBB Buffer to eliminate PCR inhibiting compounds within the samples and elute highly concentrated DNA. Purified DNA is suitable for PCR, restriction digestion and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time. Multiple samples can be processed in parallel.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Starting Amount | 25-30 mg tissue, 250 µL fecal sample / serum / urine/blood, |
| Starting Material | tissue, urine, serum, and fecal sample |
| Elution Volume | 15-100 μL |
| Technology | Silica MicroElute spin column |
| Processing Mode | Manual, Centrifugation/Vacuum |
| Note | Host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA, and viral DNA and RNA |
Kit Components
| Item | Available Separately |
|---|---|
| Disruptor Tubes | View Product |
| MicroElute® LE DNA Columns | View Product |
| 2 mL Collection Tubes | /product/2-0ml-tube-cap-less-graduated-100-pk-50pk-cs/">View Product |
| SLX-Mlus Buffer | View Product |
| DS Buffer | Call for Pricing |
| PCP Buffer | --- |
| RBB Buffer | View Product |
| HBC Buffer | View Product |
| DNA Wash Buffer | View Product |
| Elution Buffer | View Product |
| Proteinase K Solution | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
D4035 E.Z.N.A.® Universal Pathogen Kit
SDS
D4035 SDS
SALES SHEET
Product Data
Using E.Z.N.A.® Universal Pathogen Kit on various samples produced better quality DNA.
Figure 1. Human fecal samples suspended in PBS solution were spiked into corresponding organisms. Fecal samples were then processed according to each manufacturer’s recommended protocols. qPCR was performed in triplicate for each sample using primers specific for the target organism. Data shown are averages on triplicate reactions.
Citations
- Lin, Qiuyuan, et al. “Real-Time Fluorescence Loop-Mediated Isothermal Amplification Assay for Rapid and Sensitive Detection of Streptococcus Gallolyticus Subsp. Gallolyticus Associated with Colorectal Cancer.” Analytical and Bioanalytical Chemistry, vol. 411, no. 26, 6 Aug. 2019, pp. 6877–6887, 10.1007/s00216-019-02059-8. Accessed 1 June 2020.
