ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
CCL21; 6Ckine; CKb9; ECL; SCYA21; TCA4; ECL; Chemokine(C-C-Motif)Ligand 21; Beta Chemokine Exodus-2; Efficient Chemoattractant For Lymphocytes
- Product No.SEB575Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.7pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 466 For more details, please contact local distributors!US$ 665 For more details, please contact local distributors!US$ 2993 For more details, please contact local distributors!US$ 5653 For more details, please contact local distributors!US$ 46550 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
This assay has high sensitivity and excellent specificity for detection of Secondary Lymphoid Tissue Chemokine (SLC).No significant cross-reactivity or interference between Secondary Lymphoid Tissue Chemokine (SLC) and analogues was observed.
Recovery of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
Matrices listed below were spiked with certain level of recombinant Secondary Lymphoid Tissue Chemokine (SLC) and the recovery rates were calculated by comparing the measured value to the expected amount of Secondary Lymphoid Tissue Chemokine (SLC) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-104 | 99 |
| EDTA plasma(n=5) | 90-99 | 93 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secondary Lymphoid Tissue Chemokine (SLC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 82-90% | 96-104% | 83-101% |
| EDTA plasma(n=5) | 89-103% | 87-94% | 93-101% | 94-101% |
| heparin plasma(n=5) | 81-94% | 93-103% | 79-92% | 88-102% |
Stability of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secondary Lymphoid Tissue Chemokine (SLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secondary Lymphoid Tissue Chemokine (SLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APB575Hu01 | Active Secondary Lymphoid Tissue Chemokine (SLC) | Cell culture; Activity Assays. |
| RPB575Hu01 | Recombinant Secondary Lymphoid Tissue Chemokine (SLC) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB575Hu01 | Polyclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC) | WB; IHC; ICC; IP. |
| LAB575Hu71 | Biotin-Linked Polyclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC) | WB; IHC; ICC. |
| MAB575Hu22 | Monoclonal Antibody to Secondary Lymphoid Tissue Chemokine (SLC) | WB; IHC; ICC; IP. |
| SEB575Hu | ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB575Hu | CLIA Kit for Secondary Lymphoid Tissue Chemokine (SLC) | Chemiluminescent immunoassay for Antigen Detection. |
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—DNA转染试剂Polyjet-适用于普遍的哺乳动物细胞
LipoD293-适用于悬浮细胞的转染（包括昆虫细胞SF9）-对于普遍的哺乳动物细胞具有更优异的转染效率
—DNAandsiRNA转染试剂
Lipojet-适用于大多数哺乳动物细胞的转染-低毒性（无需换液）-用量少-DNA/RNA共转染（co-transfection）-效果优于Lipofectamine2000
—siRNA转染试剂
GenMute-适用于大多数哺乳动物细胞转染-低毒性（无需换液）-用量少-DNA/siRNA共转染（co-transfection）PepMute-适用于普遍的哺乳动物细胞的转染
而且直接使用自制的PEI非常便宜，在293上远比商品化的脂质体要好。
另外，如果你们实验室确实钱多，不怕花钱，建议你取用Promega的FugenHD,那个转染效率比脂质体更好，而且毒性小，至于价格。。。。。。。。。。。也更高。。。。。。。。。
另外，你说漂浮的细胞有表到GFP，那个不一定是真的GFP，很多时候
当然，如果考虑成本这块的话，义翘sinofection可以考虑哦~
有战友对比过罗氏X-tremeGENEDNATransfectionReagent和lipofectamine3000的优劣吗？求解答
近期，上海公卫临床研究中心的一位研究生应用两种转染试剂Turbofecttransfectionreagent（Thermo）和EntransterTM-R4000（Engreen）进行了一次RNA转染比较。比较情况如下：
实验方法
转染试剂：Turbofecttransfectionreagent（Thermo）和EntransterTM-R4000（EngreenBiosystemCo,Ltd）
待处理细胞：humanCD8+T细胞
1.针对Turbofect转染的方法
每孔培养体积均为100μL，细胞数目在105左右。
取0.5μLagomir，加入9.5μL无血清RPMI1640，充分混匀；
取0.2μLTurbofecttransfectionreagent和agomir稀释液充分混合。
转染复合物制备完成；混匀后室温下孵育15-20分钟，直接取10μL加入已铺好细胞的孔中，继续培养24h收取细胞，用PBS洗涤3次以上，以备RNA抽提。
转染Mix的配制：100nMago/N.C.：（0.5μLagomir+0.2μLTurbofect+9.3μL无血清的RPMI1640）×2.5=1.25+0.5+23.25
2.EntransterTM-R4000转染
每孔培养体积均为100μL，细胞数目在105左右。
取0.5μLagomir，加入9.5μL无血清RPMI1640，充分混匀，制成10μLagomir稀释液；
取0.25μLEntransterTM-R4000，然后加入9.75μL无血清稀释液体，充分混匀，制成10μLEntransterTM-R4000稀释液；
将EntransterTM-R4000稀释液和agomir稀释液充分混合（可用振荡器或加样器吹吸10次以上），室温静置15分钟。
转染复合物制备完成；
将20μL转染复合物加入孔中的细胞悬液中，前后移动培养皿，混合均匀；
转染后6h观察细胞状态，并更换培养基，继续培养24h后收取细胞，用PBS洗涤3次以上，以备RNA抽提。
转染Mix的配制：100nMago/N.C.：（0.5μLagomir+9.5μL无血清的RPMI1640）×2.5=1.25+23.75；Etranster稀释液：（0.25μLEtranster+9.75μL无血清的RPMI1640）×5=1.25+48.75（室温静置5分钟），取20μL至ago和N.C.管中，室温静置15分钟。
实验结果
结论：从目的miRNA表达水平的检测结果来看，转染24h后，英格恩生物公司（EngreenBiosystem）的EntransterTM-R4000(ago/NC:422912倍)转染试剂的效果优于Turbofect转染试剂(ago/NC:285870倍)。
讨论：从实验结果来看，英格恩生物公司（EngreenBiosystem）的EntranstenTM-R4000(ago/NC:422912倍)转染试剂的效果优于Turbofect转染试剂(ago/NC:285870倍)。EntransterTM-R4000是英格恩生物（EngreenBiosystem）最新研发合成的针对siRNA、microRNA、mimic、inhibitor、mRNA和shRNA等RNA的转染试剂。EntransterTM-R4000不仅可以转染小RNA，而且针对mRNA等长链RNA优化。该试剂可将RNA导入多种细胞系，包括原代细胞和悬浮细胞。无论有无血清、抗生素存在均可获得很高的转染效率。
如题，PolyplusTransfection转染试剂在中国区的代理商有哪些？求推荐1-2个靠谱的，谢谢！
DXY721认为：
悬浮细胞和贴壁细胞在转染过程中差别不大，主要差别在于转染后的筛选，当然如果你做的是瞬时转染就不存在筛选的问题了。
其实转染的过程很简单，问题是能不能转的进去的，转染率能有多少，转进去是否可以稳定表达目的蛋白等等。
我们也是用脂质体做悬浮细胞的转染，说明书上都有具体的操作过程，将脂质体和目的基因按比例混合，然后加到细胞悬液里就OK了，说的简单，实际上还是有一些细节要注意的，比如脂质体和目的基因混合的比例，转染的细胞数，细胞的代数，细胞的状态，有的还要求在转染的前一天传代一次，不过不要怕，这些在脂质体说明书上都有明确的说明，按照说明书做就可以了。
jinghuanlv认为：
悬浮细胞和贴壁细胞转染还是有很大不同的。
脂质体转染的原理基于电荷吸引原理，先形成脂质体-DNA复合物，散布在细胞周围，然后通过细胞的内吞作用，将目的基因导入细胞内，而脂质体复合物与贴壁细胞的接触机会比悬浮细胞高出很多倍，所以，脂质体转染时悬浮细胞的转染效率要明显低于贴壁细胞。
我们实验室转染悬浮细胞是用的电穿孔法，目前为止，悬浮细胞转染的最好方法还是电转，我们实验室用的电转仪是Bio-Rad的，使用条件是电压250V，电容975uF，效果不错，不妨一用。
内毒素是革兰氏阴性菌细胞壁(cellwall)上的特有成分，主要是脂多糖中的类脂A，在细菌被裂解时被释放出来，由于其化学结构和特性，在质粒的纯化过程中很容易混入质粒DNA一同提取出来。内毒素的存在会严重的影响质粒转染细胞的效率，此外会激活造血细胞（如B细胞、巨噬细胞等）的非特异免疫反应，造成实验的假阳性，所以转染级质粒的提取纯化必须去除内毒素。
