福州华志双量程内部校准HZY124/223SJ电子分析天平
来自 : mayitao
POLOdeliverer™3000TransfectionReagent PRODUCTDESCRIPTION POLOdeliverer™3000TransfectionReagentisasuperiorpolymer-basedformulationforthetransfectionofDNAintoeukaryoticcellsandprovidesthefollowingadvantages:l Highesttransfectionefficiencyinmanycelltypesandformatsl Simpleprocedurewithoutmediumchangel Transientandstabletransfectionofadherentandsuspensioncellsl VerylowcytotoxicityPOLOdeliverer™3000givessuperiortransfectionefficiencyforabroadrangeofcelllines,includingandnotlimitedtothefollowingcelllines:293 CHO-S(adherent) Hela HT29293A COS-1(w/oserum) PC12 NIH3T3293T COS7-L MDCK MCF7 PrimaryHumanFibroblasts(w/oserum) Vero HepG2 SHIPPINGCONDITIONS POLOdeliverer™3000TransfectionReagentisshippedatroomtemperature.
STORAGECONDITIONS POLOdeliverer™3000TransfectionReagentshouldbestoredat4ºC. STABILITY ThestabilityofPOLOdeliverer™3000rransfectionreagentisguaranteedfor6monthswhenithasbeenstoredasrecommended.POLOdeliverer™3000Reagentshouldnotbefrozen. GeneralprotocolofplasmidDNAtransfectionwithPOLOdeliverer™30001. Onedaybeforetransfection,plate3×105cellsin2mlmediumperwellofa6-wellcultureplate.Incubateat37ºC(5%CO2)overnight.Theconfluencyofcellsshouldbe60-80%whentransfection.2. Dilute2μgDNAin100μlmedium,suchasDMEM,RPMI1640,opti-MEM.3. Dilute4μlPOLOdeliverer™3000in100μlthesamemedium.4. AddPOLOdeliverer™3000solutiontotheDNAsolutiondropwiseandmixbypipetteorvortex,thenincubatedatroomtemperaturefor15minutes(37ºCincubationfor5minutesofthePOLOdeliverer™3000solutionandtheDNAsolutionseparatelybeforemixingisrecommended).5. Withoutchangingtheculturemedium,addthemixturedropbydropintothecultureandmixbygentlyrockingtheplate.6. Maintainthetransfectedcellsat37ºC,5%CO2for24-48hourswithoutmediumchange(Ifyouwant,themediumcanbechanged12–24hoursaftertransfection.). TableRecommendedconditionsfortransfectionwithPOLOdeliverer™3000*Culturevessel96-well24-well12-well6-well35-mm60-mm100-mm150-mmT25T75Surfacearea(cm2)0.3241010206014025
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