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货号: | SL100489-PC12 |
保存条件: | 4°C |
供应商: | 济南美中清水湾生物科技有限公司 |
数量: | 现货大量 |
英文名: | GenJet™InVitroDNATranfectionReagentforPC-12Cell |
保质期: | 一年 |
规格: | 毫升/支 |
Description
GenJet™DNA InVitro TranfectionReagentforPC12 cellispre-optimizedformostefficientlytransfectingPC12cells.PC12isacelllinederivedfromapheochromocytomaoftheratadrenalmedulla. PC12cellsstopdividingandterminallydifferentiatewhentreatedwithnervegrowthfactor. ThismakesPC12cellsusefulasamodelsystemforneuronaldifferentiation.
RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonPC12cells.GenJet™reagent,1.0ml,issufficientfor660transfectionsin24wellplatesor330transfectionsin6wellplates.
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger
DataSheet
GenJet™DNA InVitro TranfectionReagentforPC12 cellispre-optimizedformostefficientlytransfectingPC12cells.PC12isacelllinederivedfromapheochromocytomaoftheratadrenalmedulla. PC12cellsstopdividingandterminallydifferentiatewhentreatedwithnervegrowthfactor. ThismakesPC12cellsusefulasamodelsystemforneuronaldifferentiation.
RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonPC12cells.GenJet™reagent,1.0ml,issufficientfor660transfectionsin24wellplatesor330transfectionsin6wellplates.
SummaryofOptimalTransfectionConditions: Confluenceonthedayoftransfection Cellcultureconditions GenJet™(µl):DNA(µg)Ratio DiluentforDNAandTransfectionReagent IncubationTimetoFormGenJet™/DNAComplex PresenceofSerum/AntibioticsduringTransfection ChangeMediumAfterTransfection MaximalEfficiency TransfectionResults: ReporterGene Plasmid Efficiency(GFP%) | ~80% DMEMwith4.5g/Lglucose,10%FBS 3:1 Serum-freeDMEMwith4.5g/Lglucose 15minutesatRT Yes Yes,~5HoursAfterTransfection 48hours EGFP pEGFP-N3(CMVpromoter) 50% |
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger
DataSheet
温馨提示:不可用于临床治疗。
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发布于 : 2016-06-16
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