
Generalcloningstrain
Clonelargeinsertsandtransformlargeplasmids-uptoatleast145kbplasmidDNA.
- Achievehighefficiencieswithconvenientchemicallycompetentformat:>5×108cfu/µgpUC19DNA.
- Idealforcloning,subcloningandplasmidpreparationduetoendAandrecAmutations.
Applications
ThehighlyversatileTransforMax™EC100™E.colicompetentcellsareidealformostcloningapplications.ThecellsprovideveryhightransformationefficiencywhentestedagainstawiderangeofsupercoiledDNAsaswellasDNAdirectlyfromaligationreaction(Table1). Benefits
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Table1.ComparisonofthetransformationefficienciesofTransforMax™EC100™E. coliwithavarietyofDNAs.Transformationswereperformedusing50µlofcompetentcellsandeithersupercoiledDNAsoftheindicatedsizesora1-µlaliquotfromastandard10-µlligationreaction.Resultsshownareincfu/µgofDNAandaretheaveragetransformationefficienciesobtainedfromseveraltrials. |
Genotype
F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupG
TransforMaxEC100ElectrocompetentE.coli
- Transformationefficiencyof>1×1010cfu/µgofpUC19.
TransforMaxEC100ChemicallyCompetentE.coli
- Transformationefficiencyof>5×108cfu/µgofpUC19.
ORDERINFORMATION
IncludespUC19controlDNA.ebiomall.com






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在细菌及古细菌中,CRISPR系统共分成3 类,其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白(Cas蛋白)共同发挥作用,而Ⅱ类系统 。

