Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. These rigorously tested clonal cell lines stably express our premiumTet-On 3Gtransactivator. They allow you to induce expression of your gene of interest by adding Dox to the culture of transfected cells, and can save you up to two months of time in developing a complete Tet-controlled system.
Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. These rigorously tested clonal cell lines stably express our premium Tet-On 3G transactivator. They allow you to induce expression of your gene of interest by adding Dox to the culture of transfected cells, and can save you up to two months of time in developing a complete Tet-controlled system.
Easily create an inducible system by placing your gene under the control of PTRE3G, transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.
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在细菌及古细菌中,CRISPR系统共分成3 类,其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白(Cas蛋白)共同发挥作用,而Ⅱ类系统 。