商品信息
联系客服
郑重提醒：
无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
Specifications
Print Version
| Description | These 0610010F05Rik protein vectors can be used for high level expression of the 0610010F05Rik protein. Protein vectors are available for expressing from bacterial or mammalian cells with a variety of tags for easy purification. |
|---|---|
| SKU | 1001002 |
| Gene Name | 0610010F05Rik |
| Unit quantity | 500 ng |
| Aliases | Kiaa1841; mKIAA1841; RP23-188K3.6 |
| Accession Number | NM_027860 |
| Vector Map | pPB-C-His, pPB-His-GST, pPB-His-MBP, pPB-N-His, pPM-C-HA, pPM-C-His, pPM-N-D-C-HA, pPM-N-D-C-His |
| Appearance | Clear liquid |
| Insert Size | NM_027860:2157NM_027860:4140 |
| Storage Condition | 1 year when stored at -20°C or lower in a non-frost free freezer. |
| Storage Buffer | 10mM Tris-HCI, 1mM EDTA, pH8.0 |
| Vector Size | pPB-C-His:5246bppPB-N-His:5307bppPM-C-HA:4765bppPM-C-His:4752bppPB-His-MBP:6421bppPB-His-GST:6000bppPM-N-D-C-HA:4808bppPM-N-D-C-His:4797bp |
| Species | This gene is available from: Mouse |
| Bacterial Selection | Kanamycin |
| Accession Number | NM_027860 |
| Guarantee | abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user"s experimental conditions. |
| Disclaimer | 1) Disclaimer for Transcript Variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.2) Disclaimer for Gene Sequence: The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.3) Disclaimer for Intended Use: All of abm"s vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).4) Disclaimer for Extra Nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5" or 3" end of the target sequence which, in most cases, is innocuous to the stability/functionality of the construct.5) abm guarantees that at least 1 out of the 3 sgRNA constructs purchased in a set designed to be used with Cas9 Nuclease will result in gene knock-out due to frameshift mutations in over 50% of cells, after successful infection and drug selection. This guarantee applies to sgRNAs designed to target human, mouse or rat genes only. If knock-out is not achieved in extremely rare cases, a one-time replacement of another set of 3 targets with alternative sgRNA sequences will be provided. To qualify for this replacement, customers must examine knock-out efficiency by Surveyor assay. Before sending your inquiry, please make sure you have optimized your experiments as far as possible. This includes (where applicable) increasing and optimizing your MOI, increasing the duration of infection (up to 72 h), and carrying out clone screening before assaying for knock-out. Please also provide data to show that a reporter virus was used to optimize the MOI for your target cell line. Customers must provide adequate data to show >80% infection efficiency with a positive control, plus additional qPCR data to evaluate the level of mRNA expression.For vector transfection, please evaluate the vector transfection efficiency by detecting Cas9 or puromycin expression for the "All-in-One" vectors using qPCR, or neomycin for constructs containing only the sgRNA. In addition, please provide Surveyor Assay or Sanger Sequencing data on at least 20 isolated clones.abm limits its obligation and liability for the success of this technology to providing one replacement of any sgRNA lentivector product only. The replacement set will not be covered by the same guarantee. If these constructs are also considered to be ineffective then the gene is most likely not susceptible to sgRNA knock-out. |
Documents
- Protein Production Guideline using pPB Vectors
- Protein Vector Amplification
- Protein Vector FAQ
FAQs
| What is the difference between Retro-, Lenti-, and Adeno- viruses? | |
Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents.Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells. | |
| What are the correct concentration units for each recombinant viral particle? | |
For lentiviruses and retroviruses, they are measured in CFU/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC/mL). Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved. | |
| How long after transduction can the infection efficiency be observed? | |
You can observe transduction efficiency from 48 hours up to 5 days after infection. | |
References
- Wang, Q., Yu, H., Yu, H., Ma, M., Ma, Y., & Li, R. "miR‑223‑3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in�vitro" Molecular Medicine Reports. : (2018). DOI: 10.3892/mmr.2018.9742.
- Xu, T., He, B. S., Pan, B., Pan, Y. Q., Sun, H. L., Liu, X. X., ... & Wang, S. K. "MiR‐142‐3p functions as a tumor suppressor by targeting RAC1/PAK1 pathway in breast cancer" Journal of Cellular Physiology. : (2019).
- Zhang, B., Roosmalen, I. A. M., Reis, C. R., Setroikromo, R., & Quax, W. J. "Death receptor 5 is activated by fucosylation in colon cancer cells" The FEBS Journal 286(3):555–571 (2019). DOI: 10.1111/febs.14742.
- Zhao, Q., Zhao, S., Li, J., Zhang, H., Qian, C., Wang, H., … Zhao, Y. "TCF7L2 activated HOXA-AS2 decreased the glucocorticoid sensitivity in acute lymphoblastic leukemia through regulating HOXA3/EGFR/Ras/Raf/MEK/ERK pathway" Biomedicine & Pharmacotherapy 109:1640–1649 (2019). DOI: 10.1016/j.biopha.2018.10.046.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...
蚂蚁淘承诺
正品保证： 全球直采 在线追溯
蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
及时交付： 限时必达 畅选无忧
蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节； 同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上， 部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。
轻松采购： 在线下单 简单省事
蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价； 报价单与合同可以直接在线生成或打印；就像在京东购物一样， 您的鼠标点击几 次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。
售后申请： 耐心讲解 优质服务
蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时， 您可通过我的订单提交您的“申请售后”， 蚂蚁淘产品顾问会第一时间为您处理， 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。
2017-07-20
2016 年 5 月 2 日，河北科技大学副教授韩春雨课题组在 Nature Biotechnology 上发表了关于 NgAgo 基因编辑技术的论文，引发了科学界的强烈关注。然而，从一开始被认为是颠覆性的技术，到不久后大批科学家表示无法重复这一研究成果，现在，NgAgo 是否具有“基因编辑”功能还是个大大的问号。图片来源：Nature Biotechnology关于这一事件的最新动向还停留在今年 5 月。5 月 9 日，Nature 查看更多
>
2018-03-01
基因编辑黑科技 | 利用CRISPR/Cas9为DNA照相，新型细胞记录仪捕获DNA数据 查看更多
>
2021-07-27
北京时间2月15日0时，人类基因编辑研究委员会正式就人类基因编辑的科学技术、伦理与监管向全世界发布研究报告。报告将人类基因编辑分为基础研究、体细胞、生殖细胞/胚胎基因编辑三大部分，分别就这三方面的科学问题、伦理问题以及监管问题进行了讨论并提出相关原则。... 查看更多
>
2018-03-10
在过去的几年里，CRISPR 不断占据科技新闻的头条。专家预测，这种基因编辑技术将改变我们的地球，彻底改变社会的方方面面，以及和与我们共存的生物体。与其他基因工程技术手段相比，CRISPR（也被称为 CRISPR-cas9）更加精确、廉价、易于使用并且功能非常强大。发现于 20 世纪 90 年代早期、7 年后首次用于生物化学实验研究的 CRISPR 技术已迅速成为最受研究人员欢迎的基因编辑工具，在生物学、农业学和微生物学等领域广泛应用。 查看更多
>
2017-07-20
2016 年 5 月 2 日，河北科技大学副教授韩春雨课题组在 Nature Biotechnology 上发表了关于 NgAgo 基因编辑技术的论文，引发了科学界的强烈关注。然而，从一开始被认为是颠覆性的技术，到不久后大批科学家表示无法重复这一研究成果，现在，NgAgo 是否具有“基因编辑”功能还是个大大的问号。图片来源：Nature Biotechnology关于这一事件的最新动向还停留在今年 5 月。5 月 9 日，Nature 查看更多
>
2021-07-15
基因疗法也好，CRISPR技术也好，和基因编辑相关的产品是未来的发展方向，而这也给FDA的监管带来了新的挑战——毫无疑问，为了让这些产品顺利上市，更精准的监管政策是必须的。近日，美国FDA局长Robert Califf博士和FDA政策办公室的高级政策顾问Ritu Nalubola博士在官方博客上发表长文，对这个问题进行了详细探讨。以下是全文的主要内容。近期科学的进步让我们可以准确有效地对植物、动物、微生物的基因组进行编辑，让它们产生人类 查看更多
>
2018-06-28
巴傲得生物长期备有Bioworld抗体现货4000多种抗体可以在一周左右送到客户...上海遐锦生物科技有限公司于2019年11月29日举办全国循证医学Meta分析与网状... 查看更多
>
2021-09-15
日前，在一项新的研究中，来自英国剑桥大学的研究人员和他们的合作研究者对一种CRISPR基因编辑技术进行改进研究，并利用它发现急性髓性（AML）的新的治疗靶标。在他们的研究成果中鉴定出大量基因可能作为抗AML疗法的潜在靶标，与此同时描述了抑制这些基因中的一种，即KAT2A，破坏AML细胞的机制，以及怎样的操作可以同时不会伤害非白血病血细胞。 AML：acute myelocytic leukemia 即急性髓细胞白血病，是一类白血病... 查看更多
>
2018-04-13
环境学院始建于1978年，是国内最早开展环境类教学与科研的单位之一，是国内知名、具有国际影响的环境学科。如今，生物技术的发展为许多领域提供了新的解决方案，生物技术在环境研究、环境整治方面也将发挥重要作用。本次讲座吸引了众多环境学院的硕士、博士前来交流学习。 本次讲座圆满结束，在现场关注了吉锐公众号的每位听众都拿到了我们精心准备的小礼品。 ... 查看更多
>
2021-07-25
北京时间2月15日0时，人类基因编辑研究委员会正式就人类基因编辑的科学技术、伦理与监管向全世界发布研究报告。报告将人类基因编辑分为基础研究、体细胞、生殖细胞/胚胎基因编辑三大部分，分别就这三方面的科学问题、伦理问题以及监管问题进行了讨论并提出相关原则。... 查看更多
>
2018-12-26
Random subclone generationThe generation of DNA fragments by sonication is performed by placing a microcentrifuge tube containing the buffered DNA sample into 查看更多
>
2021-08-09
基因编辑是近年来发展起来的可以对基因组完成精确修饰的一种技术，包括基因敲除、敲入、定点突变、小片段的缺失、甚至大片段的替换等等。一方面，基因编辑可以用于研究基因及蛋白在特定生理、病理、发育等过程中所起的作用和功能，了解疾病的机理，找寻药物的靶点。另一方面，基因编辑还可能直接用于疾病的治疗。 CRISPR/Cas9系统是发展最为迅猛的基因编辑技术，它不仅效率高，适应面广，而且操作简单，周期相对较短。这让基因编辑技术的广泛应用和普... 查看更多
>
常见问题
蚂蚁淘所售产品均为正品吗？
蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑；
Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
下单后可以修改订单吗？
未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。
商品几天可以发货？
现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。
订单如何取消？
如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。
可以开发票吗？
本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。
如何联系商家？
蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，
或拨打4000-520-616，除此之外客户可在 联系我们页面找到更多的联系方式。
收到的商品少了/发错了怎么办？
同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。
退换货/维修需要多长时间？
一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。
商品咨询
crispr cas9 蛋白还表达怎么办_问答详情_CRISPR 细胞株_基因编辑...123
灰机52gOd2017-10-01
CRISPR(clustered,regularlyinterspaced,shortpalindromicrepeats)是一种来自细菌降解入侵的病毒DNA或其他外源DNA的免疫机制。
在细菌及古细菌中，CRISPR系统共分成3 类，其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白（Cas蛋白）共同发挥作用，而Ⅱ类系统 。
在细菌及古细菌中，CRISPR系统共分成3 类，其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白（Cas蛋白）共同发挥作用，而Ⅱ类系统 。
▍
品牌分类
▍
官网分类
▍
品牌问答
