Test Label: Biotin labeled primer / FAM probeTest Format: AmplifyRP® XRTInternal Control: Yes - Endogenous
AmplifyRP XRT for BN is a rapid DNA amplification and detection platform designed for testing grapevines for Phytoplasma solani Bois Noir. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify BN DNA and an endogenous DNA control at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal DNA amplification and detection system that rapidly amplifies small portions of DNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP XRT tests.
NOTE: This assay requires a fluorometer to work properly.
Limit of Detection (LOD): ~ 50 genomic copies per reaction
Specificity:
- Inclusive: Designed to detect all BN and Candidatus Phytoplasma solani isolates.
- Exclusive:
- Does not detect: Phytoplasma vitis.
- Predicted not to detect: Phytoplasma fragariae, Phytoplasma australiense, Phytoplasma asteris, Phytoplasma pruni, Phytoplasma americanum, Phytoplasma mali, Phytoplasma pyri, Phytoplasma japonicum, Phytoplasma convolvuli, or Phytoplasma prunorum.
- Technical Information
- Includes
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.
Includes:
- XRT reaction pellets for BN (48)
- Pre-filled 100 µL PD1 Pellet diluent tubes (48)
- GEB-filled mesh extraction bags (50)
- User Guide
NOTE: This assay requires a fluorometer to work properly.
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在细菌及古细菌中，CRISPR系统共分成3 类，其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白（Cas蛋白）共同发挥作用，而Ⅱ类系统 。
