Overview
The E.Z.N.A.® Gel Extraction kit uses the proprietary HiBind® spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.
- Rapid – DNA recovery from an agarose gel in 15 min
- Specialized buffer system – Visual determination of optimum DNA binding for higher yields
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 30-50 µL |
| Starting material | Agrose gel slice |
| Starting amount | Up to 25 µg DNA |
| DNA recovered | >90% recovery, 70 bp to 20 kb |
| Processing mode | Manual, centrifugation/vacuum |
| Throughput | 1-12 |
| DNA binding technology | Silica mini spin column |
| Binding capacity | 25 μg |
| Processing time | <10> |
Kit Components
| Item | Available Separately |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| Binding Buffer (XP2) | View Product |
| Elution Buffer | View Product |
| SPW Wash Buffer | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
D2500 Gel Extraction Kit
SDS
D2500 SDS
SALES SHEET
Product Data
Electrophoretic Analysis of recovered DNA from an agarose gel compared to the control
Figure 1. DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure. M: size marker; C: control; O: Omega Bio-tek; Q: company Q.
Comparison of DNA fragment recovery from an agarose gel
Figure 2. DNA was purified from an agarose gel using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q. The concentration of the recovered DNA was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. The purified DNA normalized to input amount is shown above.
Citations
View Citations
- Zhao, W., Wang, J., Deng, R., & Wang, X. (2008). Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter. Journal of industrial microbiology & biotechnology, 35(3), 189-195.
- Li, C. J., Sun, H. W., Zhu, F. L., Chen, L., Rong, Y. Y., Zhang, Y., & Zhang, M. (2007). Local adiponectin treatment reduces atherosclerotic plaque size in rabbits. Journal of Endocrinology, 193(1), 137-145.
- Ye, X., Li, J., Lu, M., Deng, G., Jiang, X., Tian, Y., … & Jian, Q. (2011). Identification and molecular typing of Streptococcus agalactiae isolated from pond-cultured tilapia in China. Fisheries Science, 77(4), 623-632.
- Laurila, J., Ahola, V., Lehtinen, A., Joutsjoki, T., Hannukkala, A., Rahkonen, A., & Pirhonen, M. (2008). Characterization of Dickeya strains isolated from potato and river water samples in Finland. European Journal of Plant Pathology, 122(2), 213-225.
- Laurila, J., Ahola, V., Lehtinen, A., Joutsjoki, T., Hannukkala, A., Rahkonen, A., & Pirhonen, M. (2008). Characterization of Dickeya strains isolated from potato and river water samples in Finland. European Journal of Plant Pathology, 122(2), 213-225.
- Jiang, Y., Li, Y., Fang, S., Jiang, B., Qin, C., Xie, P., … & Li, G. (2014). The role of MALAT1 correlates with HPV in cervical cancer. Oncology letters, 7(6), 2135-2141.
- Xu, C., Zhao, J., Hu, X., & Zhang, G. (2007). Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene. Veterinary microbiology, 122(1-2), 61-71.
- Koenig, C., Hirsh, A., Bucks, S., Klinner, C., Vogel, H., Shukla, A., … & Grosse-Wilde, E. (2015). A reference gene set for chemosensory receptor genes of Manduca sexta. Insect biochemistry and molecular biology, 66, 51-63.
- Shu, Y., Du, Y., & Wang, J. (2011). Molecular characterization and expression patterns of Spodoptera litura heat shock protein 70/90, and their response to zinc stress. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 158(1), 102-110.
- Du, R., Du, R., Chen, Y. F., An, X. R., Yang, X. Y., Ma, Y., … & Qin, J. (2005). Cloning and sequence analysis of myostatin promoter in sheep: Full Length Research Paper. DNA Sequence, 16(6), 412-417.
- Cai, L., Ni, J., Xia, Y., Zi, Z., Ning, K., Qiu, P., … & Yu, X. (2012). Identification of an emerging recombinant cluster in porcine circovirus type 2. Virus research, 165(1), 95-102.
- Xuan, K., Jin, F., Liu, Y. L., Yuan, L. T., Wen, L. Y., Yang, F. S., … & Jin, Y. (2008). Identification of a novel missense mutation of MSX1 gene in Chinese family with autosomal-dominant oligodontia. Archives of oral biology, 53(8), 773-779.
- Chen, R., Guo, L., & Dang, H. (2011). Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18. World Journal of Microbiology and Biotechnology, 27(2), 431-441.
- Liu, J. M., Xin, X. J., Li, C. X., Xu, J. H., & Bao, J. (2012). Cloning of thermostable cellulase genes of Clostridium thermocellum and their secretive expression in Bacillus subtilis. Applied biochemistry and biotechnology, 166(3), 652-662.
- Farnelid, H., Bentzon-Tilia, M., Andersson, A. F., Bertilsson, S., Jost, G., Labrenz, M., … & Riemann, L. (2013). Active nitrogen-fixing heterotrophic bacteria at and below the chemocline of the central Baltic Sea. The ISME journal, 7(7), 1413.
- Fu, X., Li, N., Liu, L., Lin, Q., Wang, F., Lai, Y., … & Wu, S. (2011). Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV). Virus genes, 42(1), 97-109.
- X. Guo, X. Xia, R. Tang, J. Zhou, H. Zhao, K. Wang Development of a real-time PCR method for Firmicutes and Bacterioides in faeces and its application to quantify intestinal population of obese and lean pigs
- Chung-Jiang Li, Hui-Wen Sun, Fa-Liang Zhu, Liang Chen, Yuan-Yuan Rong, Yun Zhang, Mei Zhang Local adiponectic treatment reduces atherosclerotic plaque size in rabbits
- Yan Li, Xingyi Ge, Huajun Zhang, Peng Zhou, Yan Zhu, Yunzhi Zhang, Junfa Yuan, Lin-Fa Wang, Zhengli Shi Host range, prevalence, and genetic diversity of adenoviruses in bats
- Yan Jiang, Yuehi Li, Shujuan Fang, Binyuan Jiang, Changfei Qin, Pingli Xie, Guohua Zhou, Guancheng Li The role of MALAT1 correlates with HPV in cervical cancer
- Jun Meng, Jun Xu, Dan Qin, Ying Hei, Xiang Xiao, Fengping Wang Genetic and functional properties of uncultivated MCG archaea assessed by metagenome and gene expression analysis
- Huiyan Wang, Xiangmei Liu, Shuangshuang Liu, Yangyang Yu, Jianqin Lin, Jianqiang Lin, Xin Pang, Jian Zhao Development of a markerless gene replacement system for Acidithiobacillus ferrooxidans and construction of a pfkB mutant
- Hanna Farnelid, Mikkel Bentzon-Tilla, Anders F. Andersson, Stefan Bertilsson, Günter Jost, Matthias Labrenz, Klaus Jürgens, Lasse Riemann Active nitrogen-fixing heterotrophic bacteria at and below the chemocline of the central Baltic Sea
- Maria Lluch-Senar, Miquel Vallmitjana, Enrique Querol, Jaume Piñol A new promoterless reporter vector reveals antisense transcription in Mycoplasma genitalum
- André Schuster, Kenneth S. Bruno, James R. Collett, Scott E. Baker, Bernhard Seiboth, Christian P. Kubicek, Monika Schmoll A versatile toolkit for high throughput functional genomics with Trichoderma reesei
- Yongzhen Xia, Wenqiao Chu, Qingsheng Qi, Luying Xun New insights into the QuikChange™ process guide the use of Phusion DNA polymerase for site-directed mutegenesis
- Marcela Colombres, Juan Pablo Henríquez, Germán F. Reig, Jessica Scheu, Rosario Calderón, Alejandra Alvarez, Enrique Brandan, Nibaldo C. Inestrosa Heparin activates Wnt signaling for neuronal morphogenesis
- Jinwoo Kim, Jason E. Heindl, Clay Fuqua Coordination of division and development influences complex multicellular behavior in Agrobacterium tumefaciens
- Qi-Bao Sun, Lin-Feng Li, Yong Li, Guo-Jiang Wu, Xue-Jun Ge SSR and AFLP markers reveal low genetic diversity in the biofuel plant Jatropha curcas in China
- Katherine E. Wright, Charles Williamson, Stephen E. Grasby, John R. Spear, Alexis S. Templeton Metagenomic evidence for sulfur lithotrophy by Epsilonproteobacteria as the major energy source for primary productivity in a sub-aerial arctic glacial deposit, Borup Fiord Pass
- Monica Mannelqvist, Ingunn M. Stefansson, Geir Bredholt, Trond Hellem Bø, Anne M. Øyan, Inge Jonassen, Karl-Henning Kalland, Helga B. Salvesan, Lars A. AkslenGene expression patterns related to vascular invasion and aggressive features in endometrial cancer
- Ling-Fei Li, Tao Li, Yan Zhang, Zhi-Wei Zhao Molecular diversity of arbuscular mycorrhizal fungi and their distribution partners related to host-plants and habitats in a hot and arid ecosystem, southwest China
- Junping Li, Xuan Dong, Chenghuai Yang, Qihong Li, Zhizhong Cui, Shuang Chang, Peng Zhao, Kangzhen Yu, Hanchun YangIsolation, identification, and whole genome sequencing of reticuloendotheliosis virus from a vaccine against Marek’s disease
- Jin-lan Xu, An-an Peng, Huan He, Yu Yang, Xue-duan Liu, Guan-zhou Qiu A new strain Acidithiobacillus albertensis BY-05 for bioleaching of metal sulfides ores
- Claudia Muñoz-Zanzi, Meghan Mason, Carolina Encina, Marcelo Gonzalez, Sergey Berg Household characteristics associated with rodent presence and Leptospira infection in rural and urban communities from southern Chile
- Qin-Ping Zhao, Shu Huan Zhang, Zi-Rong Deng, Ming-Sen Jiang, P. Nie Conservation and variation in mitochondrial genomes of gastropods Oncomelania hupensis and Tricula hortensis, intermediate host snails of Schistosoma in China
- Claudia Muñoz-Zanzi, Meghan R. Mason, Carolina Encina, Angel Astroza, Alex Romero Leptospira contamination in household and environmental water in rural communities in southern Chile
- Mukesh Kumar, Mansi Verma, Rup Lal Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India
- Jiansong Ju, Shujing Xu, Jianxin Wen, Gang Li, Kouhei Ohnishi, Yanfen Xue, Yanhe Ma Characterization of endogenous pyrioxal 5′-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4
- Qinghua Zhu, Jun Li, Junying Ma, Minghe Luo, Bo Wang, Hongbo Huang, Xinpeng Tian, Wenjun Li, Si Zhang, Changsheng Zhang, Jianhua Ju Discovery and engineered overproduction of antimicrobial nucleoside antibiotic A201A from the deep-sea marine actinomycete Marinactinospora thermotolerans SCSIO 00652
- Kai Qi, Jian-Jiang Zhong, Xiao-Xia Xia Triggering respirofermentative metabolism in the crabtree-negative yeast Pichia guilliermondii by disrupting the CAT8 gene
- Jeffrey P. Holt, Andrew J. Grant, Christopher Coward, Duncan J. Maskell, Jennifer J. Quinlan Identification of CJ1051C as a major determinant for the restriction barrier of Campylobacter jejuni strain NCTC11168
- Redescription and resurrection of Pachymatisma normani (Demospongiae: Geodiidae), with remarks on the genus Pachymatisma Paco Cárdenas, Joana Xavier, Ole Secher Tendal, Christoffer Schander, Hans Tore Rapp
- Márta Lőrincz, Attila Cságola, Imre Biksi, Levente Szeredi, Ádám Dán, Tamás Tuboly Detection of porcine circovirus in rodents — short communication
- Jun-Bo Yang, Jiang Yang, Hong-Tao Li, Yu Zhao, Shi-Xiong YangIsolationg and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method
- Lori A. Phillips, Valerie Ward, Melanie D. Jones Ectomycorrhizal fungi contribute to soil organic matter cycling in sub-boreal forests
- Mathieu Pruvot, Susan Kutz, Frank van der Meer, Marco Musiani Pathogens at the livestock-wildlife interface in western Alberta: does transmission route matter?
- Natalie Rose Dow Genetic variability of bovine viral diarrhea virus in persistently infected cattle
