
LP4KisLipidbasedTransfectionReagent.Itisformulatedforthehightransfectionefficiencyinmostcelltypes,bothadherentandsUSPensioncells,bestforlargeplasmidormultipleplasmidtransfection,likelentivirusproduction. SeeProductManual(.pdf).
A14.3kbexpressionlentivector(CAT#:LVP390)andthreepackagingplasmidswere transfectedintoHEK293-Tcell (0.5ugDNA/ 2ulofLP4K).Imagewastakenat12hour posttransfection
KeyFeatures:
- Bestforthetransfectionoflargeplasmidormultipleplasmidmixture;
- NoneedforDNA/Lipidratiooptimization,simplyuse1.5ulofLP4kreagentper500ngofDNAinserum-freeculture,or2.0ulofLP4Kper500ngofDNAinserumculture;
- Hightransfectionefficiencyinmostcelltypes,bothadherentandsuspensioncells;
- Hightransfectionefficiencyinbothserum-containingandserum-freemedia;
- Consistentperformancewithhighlyreproducibility
Amount: 1.0ml/pervial
Cat#:LP4K
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在细菌及古细菌中,CRISPR系统共分成3 类,其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白(Cas蛋白)共同发挥作用,而Ⅱ类系统 。

