
Background
InhibitsautophagybyblockingautophagosomeformationviainhibitionoftypeIIIphosphatidylinositol3-kinases(PI-3K)1,2.Protects1321N1astrocytomacellsagainstpyocyaninandhydroxyphenazine-inducedtoxicity3.Inhibitionofautophagyby3-MAleadstoenhancedapoptosisinducedbyavarietyofagentsincludingcelastrol4,cisplatin5andsorafenib6inseveraldifferentcelllines.Cellpermeable.
Application
Autophagosomeinhibitor
ProductInformation
Purity: >98%byTLC
MW: 149.2
Formula: C6H7N5
CASNo. 5142-23-4
PhysicalState: Lyophilizedwhitepowder
Amount: 25mg,50mg,100mg
Solubility: 3mg/mlinDMSO;10mg/mLinwarmDMF;5mg/mLinwater
Storage: Storedesiccatedassuppliedat-20°Cforupto1year.Storesolutionsat-20°Cforupto3months.
References
1.POSeglenandPBGordonetal.Proc.Natl.Acad.Sci.USA198279:1889
2.EFBlommartetal.Eur.J.Biochem.1997243:240
3.AJMcFarlandetal.Arch.Toxicol.201286:275
4.XZhaoetal.Curr.Mol.Med.201414:555
5.ZZhangetal.Oncol.Lett.201510:2941
6.HHaoetal.AnticancerDrugs201627:192
Documents
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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

