
Background
Highlyselectiveinhibitorofphosphatidylinositol3-kinase(IC50=0.31,0.73,1.06and6.60µMforPI3-Kβ,PI3-Kα,PI3-KδandPI3-Kγrespectively)1.Inhibitsautophagyinrathepatocytes(IC50=10µM)2.AnextremelyusefultoolfordeterminingtheroleofPI3-Kincellularsignaling3.Cellpermeable.MorestableinsolutionthanWortmannin,andalsoblocksautophagosomeformation.
Application
InhibitsAutophagy
ProductInformation
Purity: >98%byTLC
MW: 307.4
Formula: C19H17NO3
CASNo. 154447-36-6
PhysicalState: Lyophilizedwhitepowder
Amount: 5mg,25mg
Solubility: 20mg/mLinwarmDMSO;8mg/mLinwarmethanol
Storage: Storedesiccatedassuppliedat-20°Cforupto3years.Storesolutionsat-20°Cforupto3months.
References
1.CJVlahosetal.J.Biol.Chem.1994269:5241
2.EFBlomaartetal.Eur.J.Biochem.1997243:240
3.SChasSEROt-Golazetal.J.NeuRochem.199870:2347
Documents
ebiomall.com






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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

