
Background
Semisyntheticalkaloidalanticanceragent.InducescellcyclearrestatG2/Mphasebyinhibitingmitoticspindleformation1.Inhibitsnormalmicrotubuleassemblyandinducesaberranttubulinpolymerization1.Inducesapoptosisinavarietyofcelllines2.Inhibitsautophagosomematuration3.Inhibitspalmitoylationoftubulin4.VinblastinesulfateinhibitsmicrotubuleformationandsuppressesnAChRactivitywithIC50of8.9μMinacell-freeassay,usedtotreatcertainkindsofcancer.
Application
Autophagyinhibitor:inhibitsautophagosomematuration
ProductInformation
Purity: 98%byTLC
MW: 909.1
Formula: C46H58N4O9.H2SO4
CASNo. 143-67-9
PhysicalState: Lyophilizedwhitecrystallinepowder
Amount: 5mg,25mg
Solubility: 25mg/mLinDMSO;50mg/mLinwater
Storage: Storedesiccatedassuppliedat-20°Cforupto1year.Storesolutionsat-20°Cforupto1month.
References
1.EHamelMed.Res.Rev.199616:207
2.KTsukidateetal.Am.J.Pathol.1993143:918
3.CPSatorietal.Anal.Chem.201385:11391
4.JMCaronandMHerwoodChemotherapy200753:51
Documents
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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

