TheSUMOpro3ProteinandPeptideExpressionKit maximizestheyieldofsoluble,untagged,functionalproteinswithdesiredN-termini. Whileotherprokaryoticexpressionsystemsuseengineeredproteasecleavagesitesthatproduceaminoacidextensionsandoftenresultinnon-specificcleavagewithintheproteinofinterest, LifeSensors’highlyactiveandspecificSUMOProtease2 preciselyrecognizesthetertiarystructureoftheSUMO(hSUMO3)tag,generatingthedesiredN-terminusandnevercleavingwithinyourproteinofinterest.
BylinkingaHis6epitopetoboththeSUMO-3fusiontagandSUMOProtease2,the SUMOpro3Systemallowsaffordable,efficientandrapidpurificationofuntaggedprotein usingimmobilizedmetalaffinitychromatography.
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Info
| Fusiontag | His6-hSUMO3 |
| Promoter | T7RNApolymerase |
| SelectionMarker | Kanamycin |
| Host | E.coli |
| DNA | Circular |
Documents
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

