Background
BrefeldinAisafermentationproductfromPenicilliumbrefeldianum.ItisalactoneantibioticandATPaseinhibitorforproteintransportwithIC50of0.2μMinHCT116cells.BrefeldinAhasbeenwidelyusedasspecificinhibitorofproteintranslocationfromendoplasmicreticulumtoGolgi1.Blockssecretionandpreventstheassemblyofnon-clathrin-coatedbudsonGolgicisternae2.Inhibitsguaninenucleotide-exchangeproteinsBIG1andBIG2,activatorsofADP-ribosylationfactors3.Inducesapoptosisindependentlyofp534.Activatessphingomyelinmetabolism5.Cellpermeable.
Application
Autophagyinducer
ProductInformation
Purity: >99.0%byHPLC
MW: 280.4
Formula: C16H24O4
CASNo. 20350-15-6
PhysicalState: Lyophilizedpowder
Amount: 5mg,25mg,50mg
Solubility: 100mg/mLinDMSO;or25mg/mlinwarmethanol
Storage: Storedesiccatedassuppliedat-20°Cforupto2years.Storesolutionsat-20°Cforupto1month.
References
1.TFujiwaraetal.J.Biol.Chem.1988263:18545
2.LOrcietal.Cell199164:1183
3.KFXuetal.Proc.Natl.Acad.Sci.USA2005102:2784
4.RGShaoetal.Exp.CellRes.1996227:190
5.CMLinardicetal.CellGrowthDiffer.19967:765
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

