Ineukaryotes,endogenousSUMOproteasesrapidlycleaveSUMOfusionsinvivo,precludingtag-enhancedexpressionandpurificationcapacities.Tocircumventthisproblem,LifeSensorshasengineeredtheSUMOstarfusionsystem.SUMOstaroffersthesamesolubilityandexpressionenhancingbenefitsastheoriginalwild-typeSUMOtag;however,thetagisnotcleavedbyendogenouseukaryoticSUMOproteases.Inaddition,LifeSensorshascreatedacognateSUMOstarproteaseforspecific recognitionandcleavageoftheSUMOstartaginvitro,liberatingtheprotein-of-interestwithadesiredN-terminalaminoacid.
ThepM-MammalianSecretorySUMOstarkitallowsforexpressionandsecretionofSUMOstarfusionproteins.
ThisproductisprotectedbyoneormoreUSorForeignpatents.Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplywiththeseterms.
Info
| FusionTag | His6-SUMOstar |
| Promoter | CMV |
| Leadersequence | IgGkappachain |
| SelectionMarker(bacterial) | Ampicillin |
| Selectionmarker(mammalian) | G418 |
| Host | Mammalian |
| DNA | Circular |
Documents
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

