The pM-secSUMOstar vector allows for expression and secretion of SUMO fusion constructs in a mammalian cell system. Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence. Proteins are directed to the secretory pathway by the mouse IgG kappa secretory signal, which precedes a His6 tag. SUMOstar fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines. Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).
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Info
| Leader sequence | kappa light chain |
| Fusion tag | His6-SUMOstar |
| Promoter | CMV |
| Selection marker (bacterial) | Ampicillin |
| Selection marker (mammalian) | G418 |
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

