SUMOstarFusionProteinTechnology
ThepY-secSUMOstarvectorenablesexpressionandsecretionofSUMOstarfusionsin Pichiapastoris.Proteinexpressionisdrivenbytheprimaryalcoholoxidase(AOX1)promoter,whichisbothtightlyregulatedandhighlyinducIBLe.Thealphamatingfactor(S.cerevisiae)targetsthefusionproteintothesecretorypathway,whilebothaFLAGandaHis6 tagpreceedSUMOstarforconvenientpurification.ChromosomalintegrationisaccomplishedbyDNAlinearizationwithinthepromoterusingavarietyofconvenientendonucleaserestrictionsites,whilemult-copyintegrantscanbeobtainedthroughstrainselectionagainstincreasingconcentrationsoftheantibioticZeocin.
Components:
- pY-secSUMOstarvector(Cat.#2161)—20ug
- SUMOstarProtease(Cat.#4110)—500Units
- SUMOstarProteasecontrolprotein(Cat.#5010)—100 µg
- Anti-SUMOstarantibody(Cat.#AB7002)—50 µg
Info
| FusionTag | His6-hSUMOstar |
| Promoter | AOX1 |
| Host | P.pastoris |
| DNA | Circular |
| Storage | Storevectorcomponentsat-20°C,storeproteaseandantibodycomponentsat-80°C,avoidrepeatedfreeze-thawcycles |
Applications
- EnhancingfunctionalproteinexpressioninP.Pastoris.
Benefits
- SUMOstarfusionoftenenhancesexpression.
- SUMOstarfusionoftenenhancesproteinsolubility.
- NoknowncaseofSUMOstarProteasecleavingwithinthefusedprotein-of-interest.
- CleavageyieldsnativeproteinwithadesiredN-terminus.
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

