ForremovalofSUMO(smt3)tagsfromfusionproteinsexpressedusingSUMOprosystems(Cat#s: 1000A, 1000K, 1001A, 1001K).LifeSensors"industryleADIngSUMOProtease1(Ulp1),ahighlyactiveandrobustrecombinantprotease,cleavesSUMOfromrecombinantfusionproteins.Unlikethrombin,EK,orTEVprotease,whichrecognizeshortlinearsequences,SUMOProtease1recognizesthetertiarystructureofSUMO.Asaresult,SUMOProtease1willnotcleavewithinthefusedproteinofinterest.
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Info
| pfam | PeptidaseC-48 |
| Molecularweight | 26kDa |
| Fusiontag | His6 |
| Source | E.coli |
| Purity | >95% |
| Physicalstate | 10U/µLin50mMHepes,pH7.5,150mMNaCl,10%glycerol |
| Activity | >105Unit/mg 1UofSUMOProtease1cleaves>90%of100ugSUMOProtease1ControlProteinin1hour(30°C) |
| Alternatenames | Ulp1core |
| Storage/StABIlity | Storeat-80°C,avoidingrepeatedfreeze-thawcycles. Stableforuptooneyearundertheseconditions. |
Applications
- DigestionandremovalofSUMOprotagsfromrecombinantfusionproteinsmadewithourSUMOproExpressionsystems (Cat#s: 1000A, 1000K, 1001A, 1001K)
Benefits
- SUMOProtease1cleavesconsistentlyoverabroadrangeoftemperature(30°Cisoptimal),pH[5.5-9.5],andionicstrength.SUMOProtease1containsapolyhistidinetagattheN-terminus,makingiteasytoremovefromthecleavagereactionbyaffinitychromatography.
Documents
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

