Smallubiquitin-likemodifier(SUMO)proteinsareafamilyofproteinsthathaveasimilarstructurewithubiquitin.TherearefourSUMOgenesinhumangenome.SUMO2andSUMO3arehighlysimilar(97%identical),whileSUMO1isquitedistinctfromothermembers.SUMO4containsaspecificproline90residue,whichpreventsitfrombeingprocessedbySUMOprotease.SUMOylationisareversIBLepost-translationalmodificationthatcovalentlyattachesSUMOtotargetproteins.SUMOylationregulatesmanycriticalcellularprocesses,includingreplication,cell-cycle,proteintransportandDNArepair.SUMOisessentialforalmostalltheeukaryotes,andderegulationofSUMOylationleadstoseveraldiseasessuchascancerandneurodegenerativediseases.Therefore,itiscriticaltounderstandtheregulationofSUMO.
| Molecularweight | 5889 Da |
| AffinityTag | Biotin |
| Amount | 200 µgat5mg/mL |
| Physicalstate | Liquid(buffer:50mMHEPES,pH7.5,150mMNaCl) |
| Storage | -20°C.Avoidrepeatedfreeze/thawcycles |
Applications
- ThebiotinSUMOproteincapturereagentisabletospecificallydetectSUMOylatedproteinincombinationwithHRP-ConjugatedStreptavidin.
- ThebiotinSUMOproteincapturereagentcouldbeusedforisolatingSUMOylatedproteinfromcellsampleswithStreptavidinresin.
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

