TUBEs: ARevolutioninPolyubiquitinIsolationandCharacterization
TheuseofTandemUbiquitinBindingEntities(TUBEs)isemergingasanindispensablestrategyforubiquitinresearch.ThefirstgenerationoftheseTUBESBIndK48-andK63-linkedtetraUbchainswithsingledigitnanomolarKds,~100to1000-foldmoretightlythanmonomericUBAs.TUBEsalsoprotectproteinsfromDUBsandtheproteasome,evenintheabsenceofinhibitorsnormallyrequiredtoblocksuchactivity.ThisallowsefficientisolationofnativepolyUbchainsandattachedproteinsfromcelllines,tissues,andorgansunderconditionsthatarelesslikelytoaltercellphysiologythanthoselistedabove.TUBE1andTUBE2haverecentlybeendemonstratedtoenrichforallpolyUbchainlinkagetypes,withoutdiscrimination,makingthesereagentsappropriateevenifthelinkagetypeisnotknownfortheproteinofinterest.
ThenextgenerationofTUBEs: LinkageSpecificIsolationofPolyUbChains
K48TUBEHFwasdevelopedtoshowenhancedselectivityforK48-linkedpolyubiquitinchains(~20nM)overallotherlinkages(>2µM).ItcanbeusedaloneorinconjunctionwithourotherTUBEproducts,especiallyK63TUBEandM1(linear)TUBEtoinvestigatepolyubiquitinchainlinkageinyoursubstrateprotein.
Note:ThenewK48TUBEHFhasacomparableaffinityasourexistingK63TUBE.BothofthembindtotheircognateUb4swithaKdof~10-20nM.
Magnetic-TUBEsareTUBEmoietiesdirectlycoupledtomagneticbeads,fortheidentificationandcharacterizationofpolyubiquitinatedproteinsbywesternblottingand/ordownstreamproteomicstudies.Magnetic-TUBEsfacilitateconvenient"one-step"pull-downofpolyubiquitinatedproteins.
Info
K48TUBEHF,Magnetic
Size:1ml
Buffer:PBS,pH7.2,20%ethanol
Storage:Stableforupto6monthsat4oC.Donotcentrifugeathighspeeds(>3000g),dryorfreezethebeads.
APPLICATIONS
IsolationandenrichmentofK48-polyubiquitinatedproteinsfromcellandtissueextracts
IsolateK48-polyubiquitinatedproteinsforproteomicstudies
Documents
BENEFITS
100-foldpreferenceforK48poly-ubiquitinchainsoverallotherlinkages
TUBEsdisplayhigheraffinitytowardspolyubiquitinsthanmostubiquitinantibodies
Avoidsoverexpressionofepitope-taggedubiquitinforpulldowns
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

