TUBEs: ARevolutioninPolyubiquitinIsolationandCharacterization
TheuseofTandemUbiquitinBindingEntities(TUBEs)isemergingasanindispensablestrategyforubiquitinresearch.ThefirstgenerationoftheseTUBEsbindK48-andK63-linkedtetraUbchainswithsingledigitnanomolarKds,~100to1000-foldmoretightlythanmonomericUBAs.TUBEsalsoprotectproteinsfromDUBsandtheproteasome,evenintheabsenceofinhibitorsnormallyrequiredtoblocksuchactivity.ThisallowsefficientisolationofnativepolyUbchainsandattachedproteinsfromcelllines,tissues,andorgansunderconditionsthatarelesslikelytoaltercellphysiologythanthoselistedabove.TUBE1andTUBE2havebeendemonstratedtoenrichforallpolyUbchainlinkagetypes,withoutdiscrimination,makingthesereagentsappropriateevenifthelinkagetypeisnotknownfortheproteinofinterest.
ThenextgenerationofTUBEs: LinkageSpecificIsolationofPolyUbChains
Fluorescein-labeled(FLR)K48TUBEHFbindsK48-linkedpoly-ubiquitinwitha100-foldpreferenceforK48overotherlinkagetypesandprovidesasensitiveandcost-effectivetoolfordeterminingtheabundanceofK48-linkedpolyubiquitininthecell,oridentifyingthelinkage-typeassociatedwithaparticularproteinofinterest.FLR-K48TUBEHFisusefulfordetectionandcharacterizationofK48-linkedpoly-ubiquitylatedproteinsby"farWestern"blottingandinsitudetection(histo-orcyto-chemicalstaining).
FLR-K48TUBEHFhasonefluoresceinmoleculeconjugatedtoasingle,definedlocationwithintheprotein.
Info
Tag: Fluorescein
Size:50µg
Buffer:PBS,pH7.2
Storage: Stableforupto6monthsat-80C.Aliquotreagentandavoidrepeatedfreeze/thawcycles.
APPLICATIONS
- "Far-Western"blotanalysis
- Histo-orcyto-chemicalstaining.Samplefixationwillrequireoptimization.
BENEFITS
- NanomolaraffinityforK48poly-ubiquitinchains
- 100-foldpreferenceforK48poly-ubiquitinchainsoverallotherlinkages
- TUBEsdisplayhigheraffinitytowardspolyubiquitinsthanmostubiquitinantibodies
Documents
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

