TandemUbiquitinBindingEntities(TUBEs)capitalizeonthelinkageofmultipleubiquitininteractingmotifs(UIMs)togeneratereagentswithhighaffinityforpolyubiquitin.BiotinK63-TUBEbindsK63-linkedpolyubiquitinwith a1000 to 10,000-fold preferenceforK63overotherlinkagetypesandprovidesasensitiveandcost-effectivetoolfordeterminingtheabundanceofK63-linkedpolyubiquitininthe cell, oridentifyingthelinkage-typeassociatedwithaparticularproteinofinterest.BasedonanengineeredpeptidedevelopedbySimsetal.,BiotinK63-TUBE consistsofmultiple ubiquitininteracting motifsseparatedbyrigidlinkers,presumablyincreasingitsaffinitytoextendedK63polyubiquitinchains.BiotinK63-TUBE isusefulfordetectionandcharacterizationofK63-linkedpolyubiquitylatedproteinsbyFarWesternblotting,affinitypurificationfromlysatesandBIOLOGicalfluids,andinsitudetection.
FarWesternswithBiotinK63-TUBE1requireonlyminormodificationstoexistingimmunoblotprotocols,themostnotableofwhichistoavoidsubjectingtheblottodenaturingconditions,e.g.boilingorautoclaving,whicharecommontreatmentsusedinWesternblottingprotocolsforubiquitinantibodies.BiotinK63-TUBEsubstitutesfortheanti-ubiquitinIgGastheprimarydetectionreagent.Secondarydetectionisthenaccomplishedwithanavidinor streptavidinenzyme conjugate.
Fluorescein-(UM504F)andTAMRA-(UM504T)labeledversionsofK63TUBEarealsoavailableforfarWesternapplications.Pleaseinquirefordetails.
ThisproductisprotectedbyoneormoreUSorForeignpatents. Bypurchasingthisproduct,thepurchaseragreestocomplywiththetermsofour LimitedUseLabelLicense.
Info
| Formulation | 0.5mg/mlinPBS,pH7.2 |
| MolecularWeight | 10,320Da |
| Recommendeddilution | 1:1000 |
Applications
- DetectionofK63-linkedpolyubiquitinylatedproteinsbyligand(farWestern)blotting
- Identificationofthepolyubiquitinlinkage-typeofyourproteinofinterest
- InhibitionofK63-dependentprocessesinlysates
- PurificationofK63-linkedpolyubiquitinylatedproteinsfromcellandtissuelysatesusingavidinsupports
- Insitu labelingfordetectionofK63-linkedpolyubiquitinylatedproteinsbyhistochemistry
Benefits
- SelectiveforK63-linkedpolyubiquitinchains
- Superiortoastandardantibody-baseddetection
- Convenientdetectionviastandardizedligandblotting(farwestern)techniques
- Highsensitivity
- Lowcostperblot
Documents
References
[1] Sims,JJ,Cooper,EM,Scavone,F,Kane,LA,Youle,RJ,Boeke,JD,andCohen,RE. (2012).“Ubiquitinsensorpeptidesreveallocalizationandlinkage-typedependenceofcellularpolyubiquitinsignaling.”NatureMethods, 9(3):303-9.
[2] Hjerpe,R,Aillet,F,Lopitz-OtsoaF,Lang,V,EnglandP,andRodriguez,MS. (2009).“Efficientprotectionandisolationofubiquitylatedproteinsusingtandemubiquitin-bindingentities.” EMBORep.,10(11):1250-8.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

