Thiskitallowsyoutodetectwhetheryourtargetproteinisubiquitylatedanddeterminethelinkagetypeoftheubiquitinchain.
Determiningthelinkageofpolyubiquitinontargetproteinsischallenging.ThetrADItionalmethodsareeitherthroughMassSpectrometrywhichiscumbersomeorimmunoblotwith linkagespecific antibodies,whichareunreliable.LifeSensorshasdevelopedamoredefinitivemethodfordemonstratingtheubiquitylationlinkageofaprotein,whichistocoupleimmunoprecipitationofpolyubiquitylatedproteinwithdigestionbya linkagespecificdeubiquitylase priortoimmunoblotanalysis. Anincreasedsignalfortheunmodifiedsubstrateoradecreasedsignalof polyubiquitylated substrateat high molecularweightafterK48/K63specificDUBtreatmentisaclearindicationthattheproteinwasK48/K63ubiquitylated. WehavebuiltthiskitaroundtheuseofMagneticTandemUbiquitinBindingEntities(TUBEs)whichbindtoallubiquitinchainlinkages.Magnetic-TUBEsareTUBEmoietiesdirectlycoupledtomagneticbeads,fortheidentificationandcharacterizationofpolyubiquitinatedproteinsbywesternblottingand/ordownstreamproteomicstudies. Magnetic-TUBEsfacilitateconvenient"one-step"pull-downofpolyubiquitinatedproteins.
ThiskitincludesthefollowingDUBs:
- ALys48linkagespecificDUB-25 µg,10 µM
- AbroadspectrumDUB-25 µg,10 µM
Applications
- Convenientone-steppull-downofpolyubiquitylatedproteinsfromcellandtissueextracts
- Isolateubiquitylatedproteinsforproteomicstudies
- Confirmationoftargetproteinubiquitylation
Benefits
- Realproofofubiquitylation–nomassspecneeded!
- Verificationthatthepatternobservedisfromubiquitin,nototherpost-translationalmodifications
- Easycomparisonofpolyubiquitinlevelsbetweentreatedsamples
- EasyscreeningformultiplePOIs
- Nosubstratepurificationneeded
- Controlforantibodynotrecognizingubiquitylatedsubstrate
- Sufficientforaminimumof10pull-downs
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

