Basedonproteindomainsknowntopossessanaffinityforubiquitin,TandemUbiquitinBindingEntities(TUBEs)havebeendevelopedfortheisolationandidentificationofubiquitylatedproteins.TUBEsdisplayuptoa1000-foldincreaseinaffinityforpoly-ubiquitinmoietiesoverthesingleubiquitinbindingassociateddomain(UBA).Inaddition,TUBEsdisplayaprotectiveeffectonpolyubiquitinatedproteins,allowingfordetectionatrelativelylowabundance.Thesepropertieseffectively“capture”proteinsintheirpolyubiquitylatedstate.
TUBE2isbasedonUBA1fromthehumanRAD23A.WehavedevelopedtheHRP-TUBE2forquickandeasydetectionofpolyubiquitinandpolyubiquitinatedsubstrates.
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Applications
- Detectionofpolyubiquitylatedproteinsbyligandblotting(usea1:1000to1:5000dilutionformostapplications)
- Insitu labelingfordetectionofpolyubiquitinbyhistochemistry
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

