TheSmt3-CHOP-ReporterDeSUMOylationAssayKitconsistsofSmt3fusedtoareporterenzyme,aswellasasubstrateforthereporterenzyme. WhenfusedtoSmt3,thereporterisrenderedcatalyticallyinactive. FollowingcleavageoftheSmt3-reportersystembyisopeptidaseactivity,thefree(andnowactive)reporterenzymesubsequentlyactsuponitsfluorescencebasedsubstrate.Thus,inthiscoupledassay,thesignalgeneratedbycleavageofthereporterenzyme’ssubstrateisaquantitativemeasureofisopeptidaseactivity.
ThisproductisprotectedbyoneormoreUSorForeignpatents. Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplywiththeseterms.
Contents:
- Smt3-CHOP-Reporter(ReporterSystem):1x220μL(4μM)
- ULP1core(ControlIsopeptidase):2x10μL(5nM)
- ReporterSubstrate:1x120μL(5mM)
Info
| Storage | -80°C,avoidfreeze/thawcycles |
Applications
- DemonstrationofnoveldeSUMOylationactivity.
- Highthroughputscreening(HTS)forantagoNISTsoragonistsofdeSUMOylationactivity.
Benefits
- RapidandrobustreadoutofdeSUMOylatingactivitywithin60min;S/Bratio>30
- Non-rADIoactivereportersubstrates
- AmenabletoHTS,aswellasminiaturizationforcosteffectivescreening
- DetectionofdeconjugationofSmt3fromamacromolecularconjugateprotein
- Readoutdoesnotrequireexcitationinthe UVrange(reducingfalsepositives)
Documents
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

