Ub-VME is synthesized by the conjugation of 4-amino-but-2-enoic methyl ester to the C-terminus of UbiquitindeltaG76. Binding of ubiquitin by deubiquitylases (DUBs), for instance UCH’s and most USPs, positions the reactive vinyl bond next to the sulfhydryl-group of the active site cysteine. Nucleophilic attack by the sulfhydryl on the vinyl bond produces a stable, covalent thioether bond between Ubiquitin and the deubiquitylase. Thus Ub-VME is a potent suicide inhibitor of DUBs.
Info
| Amount | 50 µg |
| State | Lyophilized powder |
| Purity | ≥ 95% by RP-HPLC |
| Molecular Weight | 8,604.9 Da |
Applications
- This inhibitor is useful for labeling DUBs in situ as well as preserving the integrity of polyubiquitin chains on modified proteins for analysis or purification.
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

