Recombinant ubiquitin having a single substitution of arginine for lysine at position 11.
Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one (of seven) lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation.
Info
| Source | Human recombinant |
| Form | liquid |
| Molecular weight | 8,592 Da |
| Purity | >95% by RP-HPLC |
| Affinity Tag | None |
| Concentration | 4 mg/mL |
| Buffer | 20 mM Tris, pH 7.5, 0.15 M NaCl, 10% glycerol |
| Storage | Long term storage at -80°C. Avoid multiple freeze/thaw cycles. |
| Stability | 1 year under recommended storage conditions |
Applications
- Investigation of E1-E2-E3 mediated ubiquitin ligation.
- Investigation of DUB linkage specificity.
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

