WheninitiatingstudieswithaparticularE3ligase,UBE2D3(UbcH5c)isoftenselectedduetothereportedpromiscuityofthisE2.However,itshouldbenotedthatseveralE3ligases(includingParkin)havebeenreportednottoworkwithUBE2D3.UBE2L3(UbcH7)hasbeenshowntofunctionwithseveralE3ligases,includingE6-APandParkin.UBE2N/UBE2V2functionsasaheterodimericE2enzymefortheproductionofK63-linkedubiquitinchains,andisagoodchoiceifyouknowthattheE3ofinterestbuildsthislinkagetype.ThisinformationcanbeastartingpointinmatchingE2/E3enzymepairsforyourligaseassays,butcomprehensiveprofilingisstillsorelyneeded.
The E2SelectionPanel isdesignedtoprovidethemost flexIBLeandcost-effective solutiontothisdauntingtask.Inasinglepackage,four*E2conjugationenzymesfromourcatalogcanbeselected**.Theseenzymescanbeusedasstandaloneproducts,orinconjugationwithboththeE3LITECustomizableUbiquitinLigaseKitortheE2ProfilingKit.
*ToordermorethanfourE2s,pleaseinquireabouttheprice.
**SeveralE2sareavailablewithHis6SUMOtagorwithouttag.
***AppropriatedatasheetsforselectedE2enzymeswillbeincludedwithpurchase
Thisproductispatented. Please clickhere tolearnmore.
Info
| PanelContents | EachE2SelectionPanelcomeswith(4)E2ConjugatingEnzymes(0.8nmoleach) |
| Storage | Allcomponentsshouldbestoredat-80°C |
| StABIlity | Allcomponentsarestablefor6monthsunderrecommendedstorageconditions |
Applications
- IdentifycognateE2enzymesforE3ligases
- DemonstrationofnovelE2orE3activitywithcognateenzyme
- High-throughputscreening(HTS)ofagoNISTs/antagonistsofeitherE2orE3activity
- DeterminationofMichaelisconstant(Km)forE2:E3interactions(SeeE2:E3KineticApplicationNote)
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

