The26Sproteasomeplaysacentralroleintheubiquitin-mediateddegradationofcellularproteins.Itconsistsofa20Scatalyticcorecappedbyoneortwo19Sregulatoryparticles.Therepeat-containingdomainsofribophorins1and2(Rpn1andRpn2,110and104kDa,respectively)representthelargestsubunitsofthe26Sproteasomeandmayalsobeinvolvedinribosomebinding.Deletionofeithersubunitislethal,andmutationsinyeastresultinimpairedproteasomefunctionandaccumulationofpolyubiquitinatedproteins.Rpn1isacomponentofthe26Sproteasomebase.Inhuman,theRpn1subunitinteractswiththeubiquitinproteinligase(E3)KIAA10.RPN1andRPN2formthereceptorsfortheubiquitin-likeproteinsRad23andDsk2.Theleucine-rich-repeat-likedomainofRPN1mayparticipateintherecognitionofthecargoproteinscarriedbyRad23forunfoldingandsubsequentdegradation.ThedeubiquitinatingenzymeUbp6/USP14recognizestheproteasomebaseviatheRpn1subunit.DeubiquitinationbyUbp6preventsRpn-mediatedtranslocationtothe20Sparticle.
Info
Source | Yeastrecombinant |
Buffer | 20mMsodiumphosphatepH7.4,500mMNaCl,200mMimidazole,1mMβ-mercaptoethanol |
Molecularweight | 104.2kDa |
Expressionhost | E.coli |
Tag | His6 |
Storage | -80°C.Avoidrepeatedfreeze/thawcycles |
Applications
- Measuringthechymotrypsin-likepeptidaseactivityofthe20Sproteasome.
Documents
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

