UBE2N(homologoustoyeastUbc13)isanE2conjugatingenzymethatformsaheterodimericcomplexwiththeubiquitinE2variant(UEV)UBE2V2(alsoknownasMMS2orUEV-2).ThiscomplexisresponsIBLefortheubiquitylationoftargetsubstratesalongwithE1andE3enzymes.TheUBE2N/UBE2V2complexassemblesK63-linkedpolyubiquitinchainsthatareutilizedinnon-degradativeubiquitinsignalingpathways(DNArepair,signaltransduction).TheUEVproteinsarestructurallysimilartoE2enzymesdisplayingthesamefold,butlackingtheactivesitecysteineoftheE2.TheUBE2N/UBE2V2complexisuniqueinthatitishastheABIlitytoformfreeK63-linkedpolyubiquitinchainsinsolutionintheabsenceofanE3ligase.
Info
| Amount | 0.8nmol |
| State | Liquid |
| MolecularWeight | 17,137.9Da |
| Source | HumanRecombinant |
| FusionTag | None |
| AccessionNumber | P61088 |
| AlternateNames | UBC13,MGC8489,UbcH-ben |
| Storage | -80°C,avoidfreeze/thawcycles |
| Stability | 1yearunderrecommendedstorageconditions |
| RecommendedUse | Enzymeconcentrationof100nM–1mMisrecommendedfor invitro conjugation. |
Applications
- IdentifycognateE2/E3pairs
- Characterizeupregulation/downregulationofE2enzymes
- Buildpoly-ubiquitinchains
- CharacterizeubiquitinchainspecificityofE2enzymes
- AnalyzeexpressionofE2enzymes
Benefits
- Untagged(native)E2conjugationenzyme
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

