UBE2A is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UBE2A is a substrate for cyclin-dependent kinase 2 with phosphorylation of Ser120 dramatically increasing ubiquitin conjugating activity. During the cell cycle, peak phosphorylation of UBE2A coincides with peak ubiquitylation of histone H2B. Ubiquitylated H2B stimulates methylation of lysine 4 of histone H3. UBE2A and B are also involved in DNA lesion repair via recruitment of alternative polymerases to DNA. UBE2A has been implicated in regulating the ubiquitination of aggregating proteins along with UBE2K and UBE2D2.
Info
| Amount | 0.8 nmol |
| State | Liquid |
| Molecular Weight | 18,309.5 Da |
| Source | Human Recombinant |
| Fusion Tag | His6 |
| Accession Number | P49459 |
| Alternate Names | Rad6A, Ubiquitin-conjugating enzyme E2A |
| Storage | -80°C, avoid freeze/thaw cycles |
| Stability | 1 year under recommended storage conditions |
| Recommended Use | Enzyme concentration of 100 nM – 1 mM is recommended for in vitro conjugation. |
Applications
- Identify cognate E2/E3 pairs
- Characterize upregulation/downregulation of E2 enzymes
- Build poly-ubiquitin chains
- Characterize ubiquitin chain specificity of E2 enzymes
- Analyze expression of E2 enzymes
Benefits
- Untagged (native) E2 conjugation enzyme
Documents
ebiomall.com
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

