Crimean-CongohemorrhagicfevervirusisanegativestrandRNAvirusspreADIngacrosslargeportionsofAsia,Africa,andEurope[1]. TheLproteinofthisvirushasbeenfoundtocontainanN-terminaldomainhomologoustotheovariantumorproteasesuperfamily(OTU). TheproteasehasbeenshowntocleaveUbiquitin-,ISG15-,andNEDD8-conjugates. ThisDUBhasexhibitsstrongactivityagainstallubiquitinchainlinkagesexceptM1-,K27-,andK29-[2].
Info
Purity >95%byRP-HPLC
MolecularWeight 20,836.5Da
PhysicalState Liquid,PBSwith5%glycerol
Quantity 25 mg
| Storage | Storeat-80°C |
Documents
References
1.Capodagli,G.C.,McKercher,M.A.,Baker,E.A.,Masters,E.M.,Brunzelle,J.S.,andPegan,S.D.,Structuralanalysisofaviralovariantumordomainproteasefromthecrimean-congohemorrhagicfevervirusincomplexwithcovalentlybondedubiquitin.JournalofVirology.85,3621-3630(2011).
2.Ritorto,M.S.,Ewan,R.,Perez-Oliva,A.B.,Knebel,A.,Buhrlage,S.J.,Wightman,M.,Kelly,S.M.,Wood,N.T.,Virdee,S.,Gray,N.S.,Morrice,N.A.,Alessi,D.R.,andTrost,M.,Screeningofdubactivityandspecificitybymaldi-tofmassspectrometry.Naturecommunications.5,4763-4763(2014).
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那糖酵解中依赖NAD的酶不是要在线粒体内反应?
蛋白酶分布广泛,主要存在于人和动物消化道中,而消化道通过口与肛门与外界相通,属于外环境或外界环境
(溶菌酶、凝血酶原等)分布在内环境
(消化)酶分布在消化道

