Highlights
- Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
- Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.
Description
| Applicable For | Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc. |
|---|---|
| Elution Volume | 50 - 200 µl |
| Equipment | Microcentrifuge |
| Purity | High quality, enzymatic-reaction ready DNA/RNA products. |
| Sample Source | DNA/RNA samples contaminated with enzymatic reaction inhibitors. |
| Sample Storage | Eluted DNA/RNA can be used immediately or stored at ≤ -20°C. |
| Type | DNA and RNA |
| Yield | 50 - 90% recovery |
Q1: What is the smallest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
The smallest volume will be 50 ul.
Q2: What is the largest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
The OneStep PCR Inhibitor Removal Kit can process about 500 µl of sample at a time. However, the same column can be reused for the same sample.
Q3: Can I centrifuge at a lower speed than 3,500 x g for the 96-well kit?
No, the kit requires the speed to be at 3,500 x g. Centrifuging at lower speeds can result in inefficient sample elution.
“The ease.Holy cow, this thing was so freaking simple compared to similar products”
-Christian H. (University of Georgia)
“It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.”
-Tara N. (United States Agricultural Research Service)
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| D6035-1-30 | Prep Solution | 30 ml | $18.00 | |
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack | $108.00 | |
| C2003 | Elution Plate | 2 Plates | $19.00 | |
| C2009 | Silicon-A-HRC Plate | 2 Plates | $405.00 |
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1、多种染料及淬灭基团自由选择。
2、专利的ZENTM或TAOTM双重淬灭
基团探针设计:
·更低的背景荧光
·增强endpoint信号强度
·较少交叉污染
多种荧光及淬灭剂用于多种实验需求
PrimeTimeqPCR探针灵敏度高,可靠性大,可用于多重或数字PCR。
淬灭基团染料有多种选择(如下图),与常见的qPCR平台兼容。
为您提供严格质控的高质量探针
所有的PrimeTime探针均通过质谱和HPLC纯化验证,不同批次间产物高度一致,为您减少后顾之忧。
双淬灭基团探针提高检测灵敏度
使用ZEN或者TAODouble-Quenched探针减少背景干扰,提高检测灵敏度。独有的内部淬火剂总是位于5’端荧光基团后9个碱基与3’端IowaBlack®淬灭剂一起作用最大化探针性能(如下图)。
与传统方法相比,可以将背景噪音降低4倍(如下图A)和信号值提高大约30%(如下图B),ZENDouble-Quenched探针给出更优的表现。
对于长探针同样高效适用
对于40碱基的长探针也可以有效淬灭,这意味着在探针设计上有更多选择,比如富含AT的区域。
希望对有需要的朋友有帮助,IDT探针合成,2073904839qq.com,www.nanodigmbio.com,
请教有没有人知道哪里可以做数字PCR(dd-PCR)的公司,急求!
跪谢!
目前各家仪器公司推出自己的数字PCR,目前市场主流的是三家,1、LIFETechnologies3D数字PCR(芯片式数字PCR):我个人认为是微孔数字PCR,主要是将20ul反应体系分散到20000个微孔中进行反应,变成20000个反应体系,PCR反应结束后,采用CCD拍照,数阳性反应孔。2、Bio-Rad的微滴数字PCR(油包水原理):将20ul反应体系采用液滴反应器形成20000个油包水反应体系,PCR反应结束后,才有流式细胞术的原理,检测每一个液体,数阳性反应的液滴数量。3、RainDance的数字PCR(油包水原理)、原理与伯乐微滴数字PCR相同,但是其液体形成能力比伯乐强很多,理论上可以产生1000万个小油滴。价格从高到底:Raindance、Bio-Rad、LIFETechnologies。目前本人使用的是Bio-RadQX200、正在做实验室,希望广数字PCR的使用者可以相互交流,相互解答疑问,更好的利用数字PCR。

