
Overview:
HIPK1orhomeodomain-interactingproteinkinase1isaser/thrproteinkinaseandamemberoftheHIPKfamily.HIPK1isanuclearkinasethatphosphorylateshomeodomaintranscriptionfactors.HIPK1phosphorylatesDAXXandthisleadstoitsrelocalizationandsubsequentdecreaseintranscriptionalrepressionactivity(1).HIPK1alsointeractswithp53andphosphorylatesitonserineresidues.HIPK1expressioniselevatedinbreastcancercelllinesandembryonicfibroblastsfromHIPK1-nullmiceshowmoresusceptibilitytoapoptosisinducedbyDNAdamage(2).
GeneAliases:
Myak,Nbak2,KIAA0630,MGC26642,MGC33446,MGC33548
GenbankNumber:
NM_152696
References:
1.Ecsedy,JA.etal:Homeodomain-interactingproteinkinase1modulatesDaxxlocalization,phosphorylation,andtranscriptionalactivity.MolCellBiol.2003Feb;23(3):950-60.2.Kondo,S.etal:Characterizationofcellsandgene-targetedmicedeficientforthep53-bindingkinasehomeodomain-interactingproteinkinase1(HIPK1).ProcNatlAcadSciUSA.2003Apr29;100(9):5431-6
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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