
Overview:
HSP70isamemberoftheheatshockproteinfamilyandissynthesizedbycellsofmanyorganismsinresponsetostress(1).HSP70isfoundmostlybutnotexclusivelyinthenucleusofunstressedcells.Forseveralhoursafterashortheatshock,however,itisstronglyconcentratedinnucleoli(2).Nucleoliaretransientlydamagedbysuchaheatshock:theirmorphologychangesandassemblyandexportofribosomesisblockedforseveralhours.HSP70helpstostABIlizethemorphologicalchangesofthenucleoli.
GeneAliases:
HSPA1A,HSP70-1,HSP72,HSPA1,HSPA1B.DAQB-147D11.1
GenbankNumber:
NM_005345
References:
1.Moran,LA.etal:Themajorheat-shockprotein(hsp70)genefamily:relatedsequencesinmouse,Drosophila,andyeast.CanJBiochemCellBiol.1983Jun;61(6):488-99.2.Pelham,HR:Hsp70acceleratestherecoveryofnucleolarmorphologyafterheatshock.EMBOJ.1984Dec20;3(13):3095-100.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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