
Overview:
HSP90alphaisamemberoftheHSP90familyofproteinswhichareimportantmolecularchaperonesinvolvedinsignaltransduction,cellcyclecontrol,stressmanagement,folding,degradation,andtransportofproteins(1).HSP90isamolecularchaperonethatplaysakeyroleintheconformationalmaturationofoncogenicsignalingproteins,includingHER2/ERBB2,AKT,RAF1,BCR-ABLandmutatedp53.HSP90inhibitorsbindtoHSP90,andinducetheproteasomaldegradationofHSP90clientproteins.HSP90alphaisanimportantmediatorofcancercellinvasionandisexpressedextracellularlyonfibrosarcomaandbreastcancercellswhereitinteractswithMMP2(2).
GeneAliases:
HSP90AA1,HSPN,LAP2,HSP86,HSPC1,HSPCA,Hsp89,Hsp90,HSP90N,HSPCAL1,HSPCAL4,FLJ31884
GenbankNumber:
NM_005348
References:
1.Csermely,P.etal:The90-kDamolecularchaperonefamily:structure,function,andclinicalapplications.PharmacolTher.1998Aug;79(2):129-68.2.Eustace,B.K.etal:Functionalproteomicscreensrevealanessentialextracellularroleforhsp90-alphaincancercellinvasiveness.NatureCellBiol.6:507-514,2004.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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