
Overview:
IKKalphaisaserine/threonineproteinkinasethatphosphorylatestheI-kappa-BproteinwhichisaninhibitorofthetranscriptionfactorNF-kappa-Bcomplex.PhosphorylationofI-kappa-Bproteintriggersthedegradationoftheinhibitorviatheubiquitinationpathway,therebyactivatingNF-kappa-Bcomplex(1).IKKalphaisanessentialregulatorofNF-kappa-B-dependentgeneexpressionthroughcontrolofpromoter-associatedhistonephosphorylationaftercytokineexposure(2).IKKalphaisacriticalcomponentofthecytoplasmictransductional-transcriptionalprocessorleADIngtoinductionofIFNalphaproduction.IKKalphaisalsoinvolvedintheepidermiswhereitantagonizesmitogenicandangiogenicsignalsandrepressestumorprogressionandmetastases.
GeneAliases:
CHUK,IKK1,IKBKA,TCF16,NFKBIKA,IKK-alpha
GenbankNumber:
BC092514
References:
1.Anest,V.et.al:AnucleosomalfunctionforI-kappa-Bkinase-alphainNF-kappa-B-dependentgeneexpression.Nature423:659-663,2003.2.Hoshino,K.et.al:I-kappa-Bkinase-alphaiscriticalforinterferon-alphaproductioninducedbyToll-likereceptors7and9.Nature440:949-953,2006.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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