
Overview:
IL-2orinterleukin2isasecretedcytokinewhichreferredtoasT-cellgrowthfactor,thatisapowerfullyimmunoregulatorylymphokinethatisproducedbylectin-orantigen-activatedTcells.IL2canactasagrowthhormonethatisimportantfortheproliferationofTandB-lymphocytes(1).IL2expressionismonoallelic,whichrepresentsanunusualregulatorymodeforcontrollingthepreciseexpressionofasinglegene(2).
GeneAliases:
TCGF;lymphokine;IL2
GenbankNumber:
NP_000577.2
References:
1.Lowenthal,J.W.et.al:Similaritiesbetweeninterleukin-2receptornumberandaffinityonactivatedBandTlymphocytes.Nature315:669-672,1985.2.Hollander,G.A.et.al:Monoallelicexpressionoftheinterleukin-2locus.Science279:2118-2121,1998.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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