
Overview:
IL-8orinterleukin8isamemberoftheCXCchemokinefamilythatisoneofthemajormediatorsoftheinflammatoryresponseandseveralcelltypessecretethischemokine.Itfunctionsasachemoattractant,andisalsoapotentangiogenicfactor.IL-8playsanessentialroleinthepathogenesisofbronchiolitis,acommonrespiratorytractdiseasecausedbyviralinfection(1).IL8isproducedandreleasedfromhumanADIposetissueandfromisolatedadipocytesinvitro,whichmayindicatethatIL8fromadiposetissuecouldbeinvolvedinsomeobesity-relatedcomplications(2).
GeneAliases:
NAF;GCP1;LECT;LUCT;NAP1;CXCL8;GCP-1;LYNAP;MDNCF;MONAP;NAP-1
GenbankNumber:
NP_000575.1
References:
1.Smyth,R.L.et.al:Theassociationbetweendiseaseseverity,cytokinesandvirusgenotypeininfantswithrespiratorysyncytialvirus(RSV)bronchiolitis.ArchDis.Child.82(Suppl.1):A4-A5,2000.2.Bruun,J.M.et.al:Regulationofinterleukin8productionandgeneexpressioninhumanadiposetissueinvitro.J.Clin.Endocr.Metab.86:1267-1273,2001.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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