
Overview:
INCENPorinnercentromereproteinantigens135/155kDaisaconstitutivelybindingcentromereproteinsand'passenger,'ortransientlyinteracting,proteins.TheoverexpressionofatruncationmutantofchickenINCENPandachimericCENPB:INCENPproteininmammaliancellculturedisruptedbothprometaphasechromosomealignmentandcompletionofcytokinesis(1).INCENPformsacomplexwiththeevolutionarilyconservedfamilyofAuroraBkinaseswhichhelpscoordinatechromosomesegregation,spindlebehavior,andcytokinesisduringmitosis(2).
GeneAliases:
FLJ31633,MGC111393
GenbankNumber:
NM_001040694
References:
1.Mackay,A.M.et.al:Adominantmutantofinnercentromereprotein(INCENP),achromosomalprotein,disruptsprometaphasecongressionandcytokinesis.J.CellBiol.140:991-1002,1998..2.Pereira,G.et.al:SeparaseregulatesINCENP-AuroraBanaphasespindlefunctionthroughCdc14.Science302:2120-2124,2003.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
向左转|向右转

