
Overview:
IP10orinterferongammainducIBLeprotein10isamemberoftheCXCchemokinefamilythatplaysaroleinpleiotropiceffects,includingstimulationofmonocytes,naturalkillerandT-cellmigration,andmodulationofadhesionmoleculeexpression.IP10participateintheregulationofangiogenesisduringinflammationandtumOrigenesis(1).IP10playsaroleinthegenerationanddeliveryofaneffectorT-cellresponse(2).
GeneAliases:
C7;IFI10;INP10;crg-2;mob-1;SCYB10;gIP-10;CXCL10
GenbankNumber:
NP_001556.2
References:
1.Angiolillo,A.L.et.al:Humaninterferon-inducibleprotein10isapotentinhibitorofangiogenesisinvivo.J.Exp.Med.182:155-162,1995.2.Dufour,J.H.et.al:IFN-gamma-inducibleprotein10(IP-10;CXCL10)-deficientmicerevealaroleforIP-10ineffectorTcellgenerationandtrafficking.J.Immun.168:3195-3204,2002.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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