
Overview:
JAK2isamemberofintracellularnon-receptortyrosinekinasesthattransducecytokine-mediatedsignalsviatheJAK-STATpathway(1).JAK2hastwonear-identicalphosphate-transferringdomains.OnedomainexhibitsthekinaseactivitywhiletheotherstABIlizestheJAKconformationalstructure.JAK2isthepredominantJAKkinaseactivatedinresponsetoseveralgrowthfactorsandcytokinessuchasIL-3,GM-CSFanderythropoietin.JAK2hasbeenfoundtobeconstitutivelyassociatedwiththeprolactinreceptorandisrequiredforresponsestogammainterferon(2).
GenbankNumber:
M_004972
References:
1.Lacronique,V.Etal:ATEL-JAK2fusionproteinwithconstitutivekinaseactivityinhumanleukemia.Science,1997;278:1309-1312.2.Ho,J.M.etal:TEL-JAK2constitutivelyactivatestheextracellularsignal-regulatedkinase(ERK),stress-activatedprotein/Junkinase(SAPK/JNK),andp38signalingpathways.Blood,2002;100:1438-1448.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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