
Overview:
MSK1ormitogen-andstress-activatedproteinkinase-1contains2proteinkinasedomainsandshares43%proteinsequenceidentitywiththeMAPKAPK1isoforms.NorthernblotanalysisshowsthatMSK1isexpressedinalltissueswiththehighestlevelofexpressioninbrain,muscle,andplacenta.ImmunoelectronmicroscopylocalizedMSK1tothenucleus.MSK1isactivatedinvitroandinvivobyeitherERKorSAPK2proteins.MSK1ratherthanMAPKAP-K1orMAPKAP-K2/K3,mediatesactivationofthecAMPresponseelement-bindingproteinandactivatingtranscriptionfactor-1byeithergrowthfactorsorstresssignals.ByrADIationhybridanalysis,theRPS6KA5genehasbeenmappedtochromosome14q31-q32.
GeneAliases:
RPS6KA5,RLPK,MSPK1,MGC1911
GenbankNumber:
NM_004755
References:
1.Deak,M.etal:Mitogen-andstress-activatedproteinkinase-1(MSK1)isdirectlyactivatedbyMAPKandSAPK2/p38,andmaymediateactivationofCREB.EMBOJ.17:4426-4441,1998.2.Jiang,C.etal:AssignmentofamemberoftheribosomalproteinS6kinasefamily,RPS6KA5,tohumanchromosome14q31-q32.1byradiationhybridmapping.Cytogenet.CellGenet.87:261-261,1999.
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提RNA一般注意:提取前材料的保存(新鲜材料,过夜处理的样品),提取中对外源性的RNA酶的清除控制(离心管,PCR管,电泳槽,台面等的无RNA酶化处理),提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒( germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
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